Extracellular DNA release by undomesticated Bacillus subtilis is regulated by early competence

Autor: Olga Zafra, José Eduardo González-Pastor, María Lamprecht-Grandio, Carolina G. de Figueras
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Genetic Screens
Gene Identification and Analysis
lcsh:Medicine
Bacillus subtilis
medicine.disease_cause
chemistry.chemical_compound
DNA transposons
Plasmid
Molecular cell biology
Genomic library
Bacterial Physiology
Cloning
Molecular
lcsh:Science
Genetics
Mutation
Multidisciplinary
Escherichia coli Proteins
Temperature
Flow Cytometry
Nucleic acids
Bacillus Subtilis
Phenotype
Prokaryotic Models
Cellular Types
Transposons
Research Article
Plasmids
Transposable element
DNA
Bacterial
Ribosomal Proteins
Gene Transfer
Horizontal
Biology
Microbiology
Molecular Genetics
Model Organisms
Bacterial Proteins
medicine
Deoxyribonuclease I
Gene Regulation
Gene
Gene Library
DNA synthesis
lcsh:R
Bacteriology
DNA
biology.organism_classification
chemistry
Prokaryotic Cells
Mutagenesis
Biofilms
DNA Transposable Elements
lcsh:Q
Cytometry
Zdroj: PLoS ONE, Vol 7, Iss 11, p e48716 (2012)
PLoS ONE
ISSN: 1932-6203
Popis: Extracellular DNA (eDNA) release is a widespread capacity described in many microorganisms. We identified and characterized lysis-independent eDNA production in an undomesticated strain of Bacillus subtilis. DNA fragments are released during a short time in late-exponential phase. The released eDNA corresponds to whole genome DNA, and does not harbour mutations suggesting that is not the result of error prone DNA synthesis. The absence of eDNA was linked to a spread colony morphology, which allowed a visual screening of a transposon library to search for genes involved in its production. Transposon insertions in genes related to quorum sensing and competence (oppA, oppF and comXP) and to DNA metabolism (mfd and topA) were impaired in eDNA release. Mutants in early competence genes such as comA and srfAA were also defective in eDNA while in contrast mutations in late competence genes as those for the DNA uptake machinery had no effect. A subpopulation of cells containing more DNA is present in the eDNA producing strains but absent from the eDNA defective strain. Finally, competent B. subtilis cells can be transformed by eDNA suggesting it could be used in horizontal gene transfer and providing a rationale for the molecular link between eDNA release and early-competence in B. subtilis that we report.
Databáze: OpenAIRE
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