Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats
| Autor: | Alexandr Klioutchnikov, Juergen Sawinski, Philip St. J. Russell, Jason N. D. Kerr, Damian J. Wallace, Richard Zeltner, Verena Pawlak, Michael H. Frosz, Kay-Michael Voit |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Optical fiber Photon Materials science Microscope Image processing Cell Biology Biochemistry law.invention 03 medical and health sciences medicine.anatomical_structure law Cortex (anatomy) Microscopy medicine Premovement neuronal activity Fiber Molecular Biology 030304 developmental biology Biotechnology Biomedical engineering |
| Zdroj: | Nature methods |
| Popis: | We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 neuronal activity for >1 h in freely moving rats performing a range of behaviors. A head-mounted three-photon microscope based on a custom-designed optical fiber and dispersion compensation enables imaging of activity from neuronal populations deep in the cortex of freely moving rats. |
| Databáze: | OpenAIRE |
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