Tomato GDSL1 is required for cutin deposition in the fruit cuticle
| Autor: | Didier Marion, Johann Petit, Anne-Laure Girard, Christophe Rothan, Jean-Luc Runavot, Bénédicte Bakan, Cédric Gaillard, Julien Vivancos, Véronique Germain, Fabien Mounet, Martine Lemaire-Chamley, Khalil Elmorjani, Bernard Quemener |
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| Přispěvatelé: | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Station de physiologie végétale, Jamin Bakan, Bénédicte |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Proteomics
0106 biological sciences ENZYMATIC-SYNTHESIS [SDV]Life Sciences [q-bio] cutine Plant Science Microscopy Atomic Force 01 natural sciences Plant Epidermis tomate Solanum lycopersicum Gene Expression Regulation Plant MOLECULAR CHARACTERIZATION biologie de la plante Research Articles Plant Proteins 2. Zero hunger 0303 health sciences Wax food and beverages [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics Plants Genetically Modified LIPASE GENE FAMILY Botanique Polyester Plant cuticle Biochemistry protéine visual_art développement du fruit visual_art.visual_art_medium RNA Interference FATTY-ACIDS Botanics expression génique Cuticle cuticule des plantes Down-Regulation ECTOPIC EXPRESSION Cutin Biology physiologie végétale Membrane Lipids 03 medical and health sciences Extracellular BIOSYNTHESIS Gene Silencing 030304 developmental biology Epidermis (botany) légume Cell Biology biology.organism_classification LIPID-TRANSFER PROTEINS PLANT BIOPOLYESTER CUTIN Fruit Waxes ARABIDOPSIS-THALIANA Solanum Carboxylic Ester Hydrolases 010606 plant biology & botany |
| Zdroj: | The Plant cell The Plant cell, American Society of Plant Biologists (ASPB), 2012, 24 (7), pp.3119-3134. ⟨10.1105/tpc.112.101055⟩ Plant Cell 7 (24), 3119-3134. (2012) |
| ISSN: | 1040-4651 1532-298X |
| DOI: | 10.1105/tpc.112.101055⟩ |
| Popis: | L'article original est publié par The American Society of Plant Biologists Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; The plant cuticle consists of cutin, a polyester of glycerol, hydroxyl, and epoxy fatty acids, covered and filled by waxes. While the biosynthesis of cutin building blocks is well documented, the mechanisms underlining their extracellular deposition remain unknown. Among the proteins extracted from dewaxed tomato (Solanum lycopersicum) peels, we identified GDSL1, a member of the GDSL esterase/acylhydrolase family of plant proteins. GDSL1 is strongly expressed in the epidermis of growing fruit. In GDSL1-silenced tomato lines, we observed a significant reduction in fruit cuticle thickness and a decrease in cutin monomer content proportional to the level of GDSL1 silencing. A significant decrease of wax load was observed only for cuticles of the severely silenced transgenic line. Fourier transform infrared (FTIR) analysis of isolated cutins revealed a reduction in cutin density in silenced lines. Indeed, FTIR-attenuated total reflectance spectroscopy and atomic force microscopy imaging showed that drastic GDSL1 silencing leads to a reduction in ester bond cross-links and to the appearance of nanopores in tomato cutins. Furthermore, immunolabeling experiments attested that GDSL1 is essentially entrapped in the cuticle proper and cuticle layer. These results suggest that GDSL1 is specifically involved in the extracellular deposition of the cutin polyester in the tomato fruit cuticle. |
| Databáze: | OpenAIRE |
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