The Role of Extracellular Vesicle and Tunneling Nanotube-Mediated Intercellular Cross-Talk Between Mesenchymal Stem Cells and Human Peripheral T Cells
| Autor: | Péter Lőrincz, Anna Brózik, Ferenc Uher, Katalin Német, Veronika S. Urbán, Zsolt Matula, Andrea Németh, Edit I. Buzás, Péter Lőw, Áron Szepesi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Cytoplasm T-Lymphocytes Biology Exosomes Lymphocyte Activation Jurkat cells Exosome Immunomodulation 03 medical and health sciences Extracellular Vesicles Jurkat Cells Cytotoxic T cell Animals Humans Microscopy Confocal Nanotubes Microvesicle Mesenchymal stem cell Cell Membrane Mesenchymal Stem Cells Cell Biology Hematology Extracellular vesicle T lymphocyte Flow Cytometry Microvesicles Coculture Techniques Cell biology Mice Inbred C57BL 030104 developmental biology Adipose Tissue Child Preschool Female Developmental Biology |
| Zdroj: | Stem cells and development. 25(23) |
| ISSN: | 1557-8534 |
| Popis: | The role of extracellular vesicles (EVs) in mediating the immunosuppressory properties of mesenchymal stem cells (MSCs) has recently attracted remarkable scientific interest. The aim of this work was to analyze the transport mechanisms of membrane and cytoplasmic components between T lymphocytes and adipose tissue-derived MSCs (AD-MSCs), by focusing on the role of distinct populations of EVs, direct cell-cell contacts, and the soluble mediators per se in modulating T lymphocyte function. We found that neither murine thymocytes and human primary T cells nor Jurkat lymphoblastoid cells incorporated appreciable amounts of MSC-derived microvesicles (MVs) or exosomes (EXOs). Moreover, these particles had no effect on the proliferation and IFN-γ production of in vitro-stimulated primary T cells. In contrast, AD-MSCs incorporated large amounts of membrane components from T cells as an intensive uptake of EXOs and MVs could be observed. Interestingly, we found a bidirectional exchange of cytoplasmic components between human AD-MSCs and primary T lymphocytes, mediated by tunneling nanotubes (TNTs) derived exclusively from the T cells. In contrast, TNTs couldn't be observed between AD-MSCs and the Jurkat cells. Our results reveal a novel and efficient way of intercellular communication between MSCs and T cells, and may help a better understanding of the immunomodulatory function of MSCs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|