Stilbene derivative as a photosensitive compound to control the excitability of neonatal rat cardiomyocytes
| Autor: | S. R. Frolova, Sandaara G. Romanova, Konstantin Agladze, Alexander M. Perepukhov, Vasili S. Gorbunov, Natalia S. Shubina |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Light Biophysics 010402 general chemistry 01 natural sciences Biochemistry Tissue Culture Techniques 03 medical and health sciences chemistry.chemical_compound Tissue culture Stilbene derivative Bromide Stilbenes Moiety Animals Humans Myocytes Cardiac Molecular Biology Ion channel Research Articles Photosensitizing Agents Photoswitch Voltage-gated ion channel Cell Biology voltage-gated channels 0104 chemical sciences Rats Quaternary Ammonium Compounds 030104 developmental biology chemistry Azobenzene Animals Newborn Neonatal rat cardiomyocyte Excitation wave propagation Research Article |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| Popis: | Substances that can be used as photosensitizers for cardiac tissue are very helpful in modeling various excitation patterns in a cardiac tissue culture and may have prospective use in the temporary and permanent ablation of unwanted excitation sources in the heart. The aim of the present work is to study the effect of stilbene derivative c-TAB (2- {4- [(E) -2- (4-ethoxyphenyl) vinyl] phenoxy} ethyl) trimethylammonium bromide) on the cardiomyocyte layers and voltage-gated ion channels in cardiac cells. C-TAB is a structural analog to AzoTAB, reported previously as a photoswitch for cardiac and neural cells, in which the azobenzene moiety is replaced by a stilbene grouping. Such a replacement makes c-TAB less toxic to living cells. c-TAB has been shown to successfully inhibit excitation in cardiac cells in both trans- and cis- forms. The excitation inhibition of cardiac cells under c-TAB is reversible and can be overturned easily by washing out the c-TAB; however, not by light illumination. The irradiation of cardiac cells with near-UV, when the trans- form of c-TAB is applied, changes reversible inhibition to a permanent one that cannot be overturned by a washout. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|