Nondestructive Assessment of Glycosaminoglycans in Engineered Cartilages Using Hexabrix-Enhanced Micro-Computed Tomography
| Autor: | Hyun Ju Oh, Long Hao Jin, Byung Hyune Choi, Xiang Yun Yin, Byoung Ju Kim, Young Jick Kim, Byoung-Hyun Min |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chemistry Micro computed tomography Cartilage Biomedical Engineering Medicine (miscellaneous) Positive correlation 030218 nuclear medicine & medical imaging Glycosaminoglycan Extracellular matrix 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Tissue engineering Engineered cartilage medicine High spatial resolution Original Article Biomedical engineering |
| Popis: | It is very useful to evaluate the content and 3D distribution of extracellular matrix non-destructively in tissue engineering. This study evaluated the feasibility of using micro-computed tomography (µCT) with Hexabrix to measure quantitatively sulfated glycosaminoglycans (GAGs) of engineered cartilage. Rabbit chondrocytes at passage 2 were used to produce artificial cartilages in polyglycolic acid scaffolds in vitro. Engineered cartilages were incubated with Hexabrix 320 for 20 min and analyzed via µCT scanning. The number of voxels in the 2D and 3D scanning images were counted to estimate the amount of sulfated GAGs. The optimal threshold value for quantification was determined by regression analysis. The 2D µCT images of an engineered cartilage showed positive correlation with the histological image of Safranin-O staining. Quantitative data obtained with the 3D µCT images of 14 engineered cartilages showed strong correlation with sulfated GAGs contents obtained by biochemical analysis (R(2) = 0.883, p |
| Databáze: | OpenAIRE |
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