Endokinin A/B stimulates rat gastric motility through myogenic NK1 receptors located in the fundus
| Autor: | Hailan Li, Shouliang Dong, Ruijia Wang, Jing Zhang, Chan Ma, Chunbo He |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Physiology medicine.medical_treatment Intraperitoneal injection Gastric motility In Vitro Techniques Pharmacology 03 medical and health sciences 0302 clinical medicine In vivo Tachykinins Physiology (medical) Pressure medicine Animals Gastric Fundus Rats Wistar Receptor Antrum Gastric emptying Chemistry digestive oral and skin physiology Antagonist Muscle Smooth General Medicine Receptors Neurokinin-1 Peptide Fragments 030104 developmental biology Gastric Emptying NK1 receptor antagonist 030217 neurology & neurosurgery Muscle Contraction Signal Transduction |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 98:691-699 |
| ISSN: | 1205-7541 0008-4212 |
| Popis: | Endokinin A/B (EKA/B), the common C-terminal decapeptide in endokinins A and B, is a preferred ligand of the NK1 receptor and regulates pain and itch. The study focused on the effects of EKA/B on rat gastric motility in vivo and in vitro. Gastric emptying was measured to evaluate gastric motility in vivo. Intragastric pressure and the contraction of gastric muscle strips were measured to evaluate gastric motility in vitro. Moreover, various neural blocking agents and neurokinin receptor antagonists were applied to explore the mechanisms. TAC4 and TACR1 mRNAs were expressed throughout rat stomach. EKA/B promoted gastric emptying by intraperitoneal injection in vivo. Correspondingly, EKA/B also increased intragastric pressure in vitro. Additionally, EKA/B contracted the gastric muscle strips from the fundus but not from the corpus or antrum. Further studies revealed that the contraction induced by EKA/B on muscle strips from the fundus could be significantly reduced by NK1 receptor antagonist SR140333 but not by NK2 receptor antagonist, NK3 receptor antagonist, or the neural blocking agents used. Our results suggested that EKA/B might stimulate gastric motility mainly through the direct activation of myogenic NK1 receptors located in the fundus. |
| Databáze: | OpenAIRE |
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