Activin promotes astrocytic differentiation of a multipotent neural stem cell line and an astrocyte progenitor cell line from murine central nervous system
| Autor: | Motonobu Satoh, Hiromu Sugino, Touho Yoshida |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Central Nervous System
endocrine system animal structures Activin Receptors Type II Smad2 Protein Biology Leukemia Inhibitory Factor Cell Line Astrocyte differentiation Mice TGF beta signaling pathway Glial Fibrillary Acidic Protein Animals Inhibins Receptors Growth Factor RNA Messenger Smad3 Protein Progenitor cell Activin type 2 receptors Smad4 Protein Neurons Lymphokines Mice Inbred C3H Interleukin-6 General Neuroscience Stem Cells Cell Differentiation Activin receptor Neural stem cell Growth Inhibitors Cell biology Activins DNA-Binding Proteins Multipotent Stem Cell Astrocytes embryonic structures Trans-Activators Neuroscience Activin Receptors Type I hormones hormone substitutes and hormone antagonists Cell Division |
| Zdroj: | Neuroscience letters. 284(3) |
| ISSN: | 0304-3940 |
| Popis: | The effects of activin A were investigated on the development of a multipotent neural stem cell line (MEB5) and an astrocyte progenitor cell line (AP-16) that were established from murine central nervous system (CNS). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis demonstrated that each cell line expresses both type I and type II activin receptors and signaling molecules for activin, Smad2, Smad3, and Smad4. Activin A did not affect the proliferation of MEB5 and AP-16 cells. When each cell line was treated alone with activin A, glial fibrillary acidic protein (GFAP), a marker for astrocytes, was induced in AP-16 cells, but not in MEB5 cells. However, activin A accelerated the leukemia inhibitory factor (LIF)-induced astroglial differentiation of MEB5 cells. These results suggest that activin promotes astrocyte differentiation of CNS neural progenitors, and the competence to activin is different between multipotent stem cells and unipotent astrocyte progenitor cells. |
| Databáze: | OpenAIRE |
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