Sub-micrometer-scale mapping of magnetite crystals and sulfur globules in magnetotactic bacteria using confocal Raman micro-spectrometry

Autor: Alexander M. Gigler, Stephan H. K. Eder, Marianne Hanzlik, Michael Winklhofer
Rok vydání: 2014
Předmět:
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 9, p e107356 (2014)
ISSN: 1932-6203
Popis: The ferrimagnetic mineral magnetite Fe3O4 is biomineralized by magnetotactic microorganisms and a diverse range of animals. Here we demonstrate that confocal Raman microscopy can be used to visualize chains of magnetite crystals in magnetotactic bacteria, even though magnetite is a poor Raman scatterer and in bacteria occurs in typical grain sizes of only 35-120 nm, well below the diffraction-limited optical resolution. When using long integration times together with low laser power (
Databáze: OpenAIRE