Proteaphagy is specifically regulated and requires factors dispensable for general autophagy
| Autor: | Kenrick A. Waite, Alicia Burris, Gabrielle Vontz, Angelica Lang, Jeroen Roelofs |
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| Rok vydání: | 2022 |
| Předmět: |
target of rapamycin (TOR)
autophagy Proteasome Endopeptidase Complex vacuole proteasome inhibitor YPD yeast-peptone dextrose starvation Ubiquitination Cell Biology yeast CP core particle Mechanistic Target of Rapamycin Complex 1 proteaphagy Biochemistry proteasome RP regulatory particle Macroautophagy protein degradation UPS ubiquitin-proteasome system Molecular Biology Research Article TORC1 target of rapamycin complex 1 |
| Zdroj: | The Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/j.jbc.2021.101494 |
| Popis: | Changing physiological conditions can increase the need for protein degradative capacity in eukaryotic cells. Both the ubiquitin-proteasome system and autophagy contribute to protein degradation. However, these processes can be differently regulated depending on the physiological conditions. Strikingly, proteasomes themselves can be a substrate for autophagy. The signals and molecular mechanisms that govern proteasome autophagy (proteaphagy) are only partly understood. Here, we used immunoblots, native gel analyses, and fluorescent microscopy to understand the regulation of proteaphagy in response to genetic and small molecule-induced perturbations. Our data indicate that chemical inhibition of the master nutrient sensor TORC1 (inhibition of which induces general autophagy) with rapamycin induces a bi-phasic response where proteasome levels are upregulated after an autophagy-dependent reduction. Surprisingly, several conditions that result in inhibited TORC1, such as caffeinine treatment or nitrogen starvation, only induced proteaphagy (i.e., without any proteasome upregulation), suggesting a convergence of signals upstream of proteaphagy under different physiological conditions. Indeed, we found that several conditions that activated general autophagy did not induce proteaphagy, further distinguishing proteaphagy from general autophagy. Consistent with this, we show that Atg11, a selective autophagy receptor, as well as the MAP kinases Mpk1, Mkk1, and Mkk2 all play a role in autophagy of proteasomes, although they are dispensable for general autophagy. Taken together, our data provide new insights into the molecular regulation of proteaphagy by demonstrating that degradation of proteasome complexes is specifically regulated under different autophagy-inducing conditions. |
| Databáze: | OpenAIRE |
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