The green microalga Lobosphaera incisa harbours an arachidonate 15S‐lipoxygenase

Autor: Ellen Hornung, B. Djian, Ivo Feussner, Till Ischebeck
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
Chloroplasts
Parietochloris incisa
Plant Science
Biology
010603 evolutionary biology
01 natural sciences
Models
Biological
positional specificity
Substrate Specificity
chemistry.chemical_compound
oxylipin formation
Complementary DNA
Catalytic Domain
Fatty Acids
Omega-3
Microalgae
Arachidonate 15-Lipoxygenase
Fatty acid hydroperoxide
Plastid
Cloning
Molecular
Ecology
Evolution
Behavior and Systematics
Phylogeny
chemistry.chemical_classification
lipid peroxidation
substrate orientation
Fatty acid
food and beverages
Stereoisomerism
General Medicine
Hydrogen-Ion Concentration
biology.organism_classification
Research Papers
Oxygen
Kinetics
Enzyme
chemistry
Biochemistry
Mutation
Arachidonic acid
lipids (amino acids
peptides
and proteins)
Heterologous expression
Oxidation-Reduction
010606 plant biology & botany
Polyunsaturated fatty acid
Research Paper
Zdroj: Plant Biology (Stuttgart, Germany)
ISSN: 1438-8677
1435-8603
Popis: The green microalga Lobosphaera incisa is an oleaginous eukaryotic alga that is rich in arachidonic acid (20:4). Being rich in this polyunsaturated fatty acid (PUFA), however, makes it sensitive to oxidation. In plants, lipoxygenases (LOXs) are the major enzymes that oxidise these molecules. • Here, we describe, to our best knowledge, the first characterisation of a cDNA encoding a LOX (LiLOX) from a green alga. To obtain first insights into its function, we expressed it in E. coli, purified the recombinant enzyme and analysed its enzyme activity. • The protein sequence suggests that LiLOX and plastidic LOXs from bryophytes and flowering plants may share a common ancestor. The fact that LiLOX oxidises all PUFAs tested with a consistent oxidation on the carbon n-6, suggests that PUFAs enter the substrate channel through their methyl group first (tail first). Additionally, LiLOX form the fatty acid hydroperoxide in strict S configuration. • LiLOX may represent a good model to study plastid LOX, because it is stable after heterologous expression in E. coli and highly active in vitro. Moreover, as the first characterised LOX from green microalgae, it opens the possibility to study endogenous LOX pathways in these organisms. peerReviewed
Databáze: OpenAIRE
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