A robotic platform for fluidically-linked human body-on-chips experimentation
| Autor: | Ben M. Maoz, Sasan Jalili-Firoozinezhad, Andrzej Przekwas, Carlos F. Ng, Alexander Cho, Anna Herland, Jose Fernandez-Alcon, Daniel Levner, Olivier Y.F. Henry, Janna Nawroth, Blakely B. O'Connor, Debarun Das, David B. Chou, Kevin Kit Parker, Anthony Bahinski, Morgan Burt, Ville J. Kujala, Susan Clauson, Mahadevabharath R. Somayaji, Thomas C. Ferrante, Ben Swenor, Henry Sanchez, Lian Leng, Tessa Huffstater, Angeliki Chalkiadaki, Miles Ingram, Donald E. Ingber, Tiama Hamkins-Indik, Norman Wen, Rachelle Prantil-Baun, Youngjae Choe, Josiah Sliz, Zachary Tranchemontagne, Bret Nestor, George J. Touloumes, Rachel Fleming, Guy Robert Thompson, Alexandra Sontheimer-Phelps, Josue A. Goss, Oren Levy, Toni Divic, Elizabeth Calamari, Sauveur S. F. Jeanty, Kyung-Jin Jang, Michael J. Cronce, Yuka Milton, Richard M. Novak, Geraldine A. Hamilton, Thomas Grevesse, Chris Hinojosa, Moran Yadid, Stephanie Dauth, John P. Ferrier, Tae-Eun Park, Robert Mannix, Edward A. FitzGerald, Aaron Delahanty |
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| Rok vydání: | 2019 |
| Předmět: |
In situ
Computer science In silico Microfluidics Inulin Cell Culture Techniques 02 engineering and technology Kidney Article 03 medical and health sciences Tissue culture chemistry.chemical_compound Lab-On-A-Chip Devices medicine Humans Fluidics Lung Skin 030304 developmental biology 0303 health sciences Brain Heart Equipment Design Robotics 021001 nanoscience & nanotechnology In vitro Intestines Coupling (electronics) medicine.anatomical_structure Liver Experimental system chemistry Blood-Brain Barrier Calibration Microscopic imaging Vascular channel 0210 nano-technology Perfusion Biomedical engineering |
| Zdroj: | Nat Biomed Eng |
| Popis: | Here we describe of an ‘Interrogator’ instrument that uses liquid-handling robotics, a custom software package, and an integrated mobile microscope to enable automated culture, perfusion, medium addition, fluidic linking, sample collection, andin situmicroscopic imaging of up to 10 Organ Chips inside a standard tissue culture incubator. The automated Interrogator platform maintained the viability and organ-specific functions of 8 different vascularized, 2-channel, Organ Chips (intestine, liver, kidney, heart, lung, skin, blood-brain barrier (BBB), and brain) for 3 weeks in culture when fluidically coupled through their endothelium-lined vascular channels using a common blood substitute medium. When an inulin tracer was perfused through the multi-organ Human Body-on-Chips (HuBoC) fluidic network, quantitative distributions of this tracer could be accurately predicted using a physiologically-based multi-compartmental reduced order (MCRO)in silicomodel of the experimental system derived from first principles. This automated culture platform enables non-invasive imaging of cells within human Organ Chips and repeated sampling of both the vascular and interstitial compartments without compromising fluidic coupling, which should facilitate future HuBoc studies and pharmacokinetics (PK) analysisin vitro. |
| Databáze: | OpenAIRE |
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