Quantitative analysis of the murine lipid droplet-associated proteome during diet-induced hepatic steatosis
| Autor: | Douglas G. Mashek, LeeAnn Higgins, Edith E. Wollaston-Hayden, Salmaan A. Khan, Todd W. Markowski |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
obesity Proteome QD415-436 Biology Proteomics liver Biochemistry Mice Endocrinology proteomics Lipid droplet medicine Animals Liver X receptor Research Articles Endoplasmic reticulum Fatty liver Lipid metabolism Lipid Droplets Cell Biology Lipid Metabolism medicine.disease Fatty Liver Mice Inbred C57BL nutrition β-oxidation Steatosis |
| Zdroj: | Journal of Lipid Research, Vol 56, Iss 12, Pp 2260-2272 (2015) |
| ISSN: | 0022-2275 |
| Popis: | Hepatic steatosis is characterized by the accumulation of lipid droplets (LDs), which are composed of a neutral lipid core surrounded by a phospholipid monolayer embedded with many proteins. Although the LD-associated proteome has been investigated in multiple tissues and organisms, the dynamic changes in the murine LD-associated proteome in response to obesity and hepatic steatosis have not been studied. We characterized the hepatic LD-associated proteome of C57BL/6J male mouse livers following high-fat feeding using isobaric tagging for relative and absolute quantification. Of the 1,520 proteins identified with a 5% local false discovery rate, we report a total of 48 proteins that were increased and 52 proteins that were decreased on LDs in response to high-fat feeding. Most notably, ribosomal and endoplasmic reticulum proteins were increased and extracellular and cytosolic proteins were decreased in response to high-fat feeding. Additionally, many proteins involved in fatty acid catabolism or xenobiotic metabolism were enriched in the LD fraction following high-fat feeding. In contrast, proteins involved in glucose metabolism and liver X receptor or retinoid X receptor activation were decreased on LDs of high-fat-fed mice. This study provides insights into unique biological functions of hepatic LDs under normal and steatotic conditions. |
| Databáze: | OpenAIRE |
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