Induction of intercellular adhesion molecule-1 (CD54) on human hepatoma cell line HepG2: influence of cytokines and hepatitis B virus-DNA transfection
| Autor: | V J Desmet, S H Yap, J. J. Van Den Oord, R Volpes |
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| Rok vydání: | 1992 |
| Předmět: |
Hepatitis B virus
Carcinoma Hepatocellular medicine.medical_treatment Immunology Intercellular Adhesion Molecule-1 Transfection medicine.disease_cause Interferon-gamma Tumor Cells Cultured medicine Humans Immunology and Allergy Autocrine signalling biology Cell adhesion molecule Liver Neoplasms biology.organism_classification digestive system diseases Cell biology Cytokine Hepadnaviridae Cell culture DNA Viral Cytokines Cell Adhesion Molecules Research Article |
| Zdroj: | Clinical and Experimental Immunology. 87:71-75 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1111/j.1365-2249.1992.tb06415.x |
| Popis: | SUMMARY Human hepatocyte expression of intercellular adhesion molecule-1 (ICAM-1)(CD54) was studied in vitro by exposing the well differentiated human hepatoblastoma cell line HepG2 to various cytokines. In addition, hepatitis B virus (HBV)-DNA transfected HepG2 cells were also analysed. Expression of ICAM-1 on HepG2 cells was then revealed with an immunohistochemical procedure. Untreated HepG2 cells were unreactive, but showed strong cytoplasmic ICAM-1 immunoreactivity after treatment with interferon-gamma (IFN-γ). This induction was completely inhibited by addition of a neutralizing antibody directed to IFN-γ, IL-1, IL-6, tumour necrosis factor-α (TNF-α) and IFN-α used alone or in combination, did not induce ICAM-1 expression, neither did they inhibit the IFN-γ-induced expression of this adhesion molecule on HepG2 cells. Untreated hepatitis B virus-DNA transfected HepG2 cells expressed membranous ICAM-1. These results indicate that IFN-γ is the main cytokine trigger for ICAM-1 expression on HepG2 cells, suggesting that in areas of liver inflammation this adhesion molecule is up-regulated on hepatocytes by locally released IFN-γ. In addition, expression of ICAM-1 by hepatitis B virus-DNA transfected HepG2 cells suggests other, still unknown, triggering mechanisms in the induction of such adhesion molecules, for instance gene activation by viral genome, or autocrine virus-induced hepatocellular cytokine production. |
| Databáze: | OpenAIRE |
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