Interleukin-8 production from cultured human dermal fibroblasts by stimulation with supernatant of cultured human epidermal cells
| Autor: | Shoso Yamamoto, Satoru Yamada, Eishin Morita, Koji Nakamura, Isamu Kimura, Yasushi Sugita |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Physiology
Neutrophils medicine.medical_treatment Inflammation Dermatology Biology medicine Humans Interleukin 8 Fibroblast Cells Cultured Chromatography High Pressure Liquid Skin Pharmacology integumentary system Epidermis (botany) Interleukin-8 Chemotaxis General Medicine Fibroblasts Molecular biology In vitro Stimulation Chemical Chemotaxis Leukocyte Cytokine medicine.anatomical_structure Epidermal Cells Cell culture Culture Media Conditioned Immunology Chromatography Gel medicine.symptom Epidermis Interleukin-1 |
| Zdroj: | Skin pharmacology : the official journal of the Skin Pharmacology Society. 6(3) |
| ISSN: | 1011-0283 |
| Popis: | There is much evidence to support the theory that keratino-cytes and dermal fibroblasts actively participate in inflammatory reactions by the production of proinflamrnatory mediators or cytokines. We investigated the neutrophil chemotactic activity in conditioned media of cultured epidermal cells and dermal fibroblasts, and found that an epidermal cell-derived factor induced fibroblasts to produce a neutrophil chemotactic factor. This neutrophil chemotactic factor was identified as interleukin-8 (IL-8) by the elution position on HPLC and by a neutralization test that uses monoclonal anti-IL-8 antibody (14E4). The epidermal cell-derived factor was fractionated together with thymocyte-proliferating activity on Sephadex G-75 gel chromatography followed by HPLC. It was blocked specifically by anti-interleukin-1 (IL-1) Α antibody, which indicates that this factor was IL-1Α. Since a variety of inflammatory dermatoses is characterized by the infiltration of neutro-phils into the skin, induction of IL-8 production in fibroblasts by epidermal cells may play an important role in inflammatory skin diseases. |
| Databáze: | OpenAIRE |
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