Macrophage plasticity is Rac signalling and MMP9 dependant
| Autor: | Andrea Parmeggiani, Farida Djouad, Mai Nguyen-Chi, Jana Travnickova, Naoill Abdellaoui, Sandra Nhim, Karima Kissa |
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| Přispěvatelé: | LPHI - Laboratory of Pathogen Host Interactions (LPHI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Laboratoire Charles Coulomb (L2C), Aigle, L2c, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Chemistry Mesenchymal stem cell Context (language use) [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular Networks [q-bio.MN] [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Matrix metalloproteinase Cell biology Extracellular matrix 03 medical and health sciences Haematopoiesis 0302 clinical medicine [NLIN.NLIN-AO] Nonlinear Sciences [physics]/Adaptation and Self-Organizing Systems [nlin.AO] [SDV.CAN] Life Sciences [q-bio]/Cancer [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular Networks [q-bio.MN] Live cell imaging 030220 oncology & carcinogenesis [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Macrophage Cellular biology Progenitor cell bioimaging [NLIN.NLIN-AO]Nonlinear Sciences [physics]/Adaptation and Self-Organizing Systems [nlin.AO] 030304 developmental biology |
| Popis: | In vitro, depending on extracellular matrix (ECM) architecture, macrophages migrate either in amoeboid or mesenchymal mode; while the first is a general trait of leukocytes, the latter is associated with tissue remodelling via Matrix Metalloproteinases (MMPs). To assess whether these stereotyped migrations could be also observed in a physiological context, we used the zebrafish embryo and monitored macrophage morphology, behaviour and capacity to mobilisation haematopoietic stem/progenitor cells (HSPCs), as a final functional readout. Morphometric analysis identified 4 different cell shapes. Live imaging revealed that macrophages successively adopt all four shapes as they migrate through ECM. Treatment with inhibitors of MMPs or Rac GTPase to abolish mesenchymal migration, suppresses both ECM degradation and HSPC mobilisation while differently affecting macrophage behaviour. This study depicts real time macrophage behaviour in a physiological context and reveals extreme reactivity of these cells constantly adapting and switching migratory shapes to achieve HSPCs proper mobilisation. |
| Databáze: | OpenAIRE |
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