Detection of Epstein-Barr genome in the lymph nodes of Hodgkin's disease
| Autor: | Francisco Mampaso, G. Fraile, T. Bricio, C. Cuesta, Carmen Bellas, Ana Ramírez de Molina |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adult
Male Herpesvirus 4 Human Pathology medicine.medical_specialty Adolescent Lymphocyte Population Biology medicine.disease_cause AIDS-Related Complex hemic and lymphatic diseases medicine Humans Reed-Sternberg Cells Child education Lymph node In Situ Hybridization Aged education.field_of_study General Medicine Middle Aged medicine.disease Hodgkin Disease Epstein–Barr virus Persistent generalized lymphadenopathy medicine.anatomical_structure Reed–Sternberg cell DNA Viral Female Lymph Nodes Lymph Generalized lymphadenopathy Research Article |
| Zdroj: | Postgraduate Medical Journal. 69:916-919 |
| ISSN: | 1469-0756 0032-5473 |
| DOI: | 10.1136/pgmj.69.818.916 |
| Popis: | Summary We have used the enzymatic in situ hybridization method to investigate the presence of Epstein-Barr virus (EBV) genome in lymph node tissues from patients with Hodgkin's disease. Also, 11 patients with persistent human immuno-deficiency virus-associated generalized lymphadenopathy as well as seven autopsy cases with no Hodgkin's disease, formed part of these studies. EBV DNA-positive reaction was demonstrated in Reed-Sternberg cells and variants in seven of 16 cases and in the small accompanying lymphocyte cell population in 14 of the 16 cases. It was also found in eight of the 11 cases with persistent generalized lymphadenopathy but in none of the lymph nodes from negative selective autopsy cases. Our results indicate that the colorimetric in situ hybridization technique is useful in EBV nucleic acid detection and cell-type localization in Hodgkin's disease. Additionally, the detection of EBV genome, not only in the diagnostic cells but in the small lymphocyte cell components, could provide new insights into the potential role of this agent in the pathogenesis of Hodgkin's disease. |
| Databáze: | OpenAIRE |
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