| Autor: |
Eric A. Bushong, T. J. Deerinck, Mark H. Ellisman, Daniela Boassa, Alice Y. Ting, Keunyoung Kim, Sebastien Phan |
| Přispěvatelé: |
Massachusetts Institute of Technology. Department of Chemistry, Ting, Alice Y |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Springer Berlin Heidelberg |
| Popis: |
The need to determine the precise subcellular distribution of specific proteins and macromolecular complexes in cells and tissues has been the major driving force behind the development of new molecular-genetic and chemical-labeling approaches applicable to high-resolution, correlated, multidimensional microscopy. This short review is intended to provide an overview of recently developed and widely used electron microscopy (EM)-compatible probes, including tetracysteine tags, mini singlet oxygen generator (MiniSOG), time-specific tag for the age measurement of proteins (TimeSTAMP) with MiniSOG, and enhanced ascorbate peroxidase (APEX). We describe how these highly specific and genetically introduced EM probes are now used, in conjunction with lower resolution light microscopic methods, to obtain wide field or dynamic records of live preparation or of large maps in 3D using recently developed laboratory-scale X-ray microscopes. The article is intended to enable researchers through a high-level view of the toolbox of labels available today for studies aiming to analyze dynamic subcellular and molecular processes in cell culture systems as well as in animal tissues—and ultimately allow investigators to determine the precise location of macromolecular complexes by EM. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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