Quantitative Proteomics Analysis of Ischemia/Reperfusion Injury-Modulated Proteins in Cardiac Microvascular Endothelial Cells and the Protective Role of Tongxinluo
| Autor: | Xiangdong Li, Jun Xu, He-he Cui, Gui-Hao Chen, Pei-Sen Huang, Qing Li, Yuejin Yang, Xia-Qiu Tian, Qiu-Ting Dong, Chen Jin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteomics Tongxinluo Physiology Cardiac microvascular endothelial cells Quantitative proteomics Myocardial Reperfusion Injury Ischemia Pharmacology Tandem mass tag lcsh:Physiology lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine medicine Humans lcsh:QD415-436 Cells Cultured Regulation of gene expression Ischemic reperfusion injury lcsh:QP1-981 business.industry Myocardium Endothelial Cells medicine.disease Ischemic/reperfusion injury 030104 developmental biology Gene Expression Regulation 030220 oncology & carcinogenesis Immunology business Reperfusion injury Drugs Chinese Herbal |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 41, Iss 4, Pp 1503-1518 (2017) |
| ISSN: | 1421-9778 |
| Popis: | Background: The protection of endothelial cells (ECs) against reperfusion injury has received little attention. In this study, we used Tandem Mass Tag (TMT) labeling proteomics to investigate the modulated proteins in an in vitro model of cardiac microvascular endothelial cells (CMECs) subjected to ischemia/reperfusion (I/R) injury and their alteration by traditional Chinese medicine Tongxinluo (TXL). Methods: Human CMECs were subjected to 2 h of hypoxia followed by 2 h of reoxygenation with different concentrations of TXL Protein expression profiles of CMECs were determined using tandem mass spectrometry. We evaluated several proteins with altered expression in I/R injury and summarized some reported proteins related to I/R injury. Results: TXL dose-dependently decreased CMEC apoptosis, and the optimal concentration was 800 µg/mL. I/R significantly altered proteins in CMECs, and 30 different proteins were detected between a normal group and a hypoxia and serum deprivation group. In I/R injury, TXL treatment up-regulated 6 types of proteins including acyl-coenzyme A synthetase ACSM2B mitochondrial (ACSM2B), cyclin-dependent kinase inhibitor 1B (CDKN1B), heme oxygenase 1 (HMOX1), transcription factor SOX-17 (SOX17), sequestosome-1 isoform 1 (SQSTM1), and TBC1 domain family member 10B (TBC1D10B). Also, TXL down-regulated 5 proteins including angiopoietin-2 isoform c precursor (ANGPT2), cytochrome c oxidase assembly factor 5 (COA5), connective tissue growth factor precursor (CTGF), cathepsin L1 isoform 2 (CTSL), and eukaryotic elongation factor 2 kinase (LOC101930123). These types of proteins mainly had vital functions, including cell proliferation, stress response, and regulation of metabolic process. Conclusions: The study presented differential proteins upon I/R injury through a proteomic analysis. TXL modulated the expression of proteins in CMECs and has a protective role in response to I/R. |
| Databáze: | OpenAIRE |
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