An Accurate and Effective Method for Measuring Osimertinib by UPLC-TOF-MS and Its Pharmacokinetic Study in Rats

Autor:	Ya-Jing Li, Song-tao Dong, Lu Meng, Yuan Zhang, Ying Li, Cong-Yang Ding, Yin Wu, Zhan-Jun Dong, Hao-Tian Yang
Rok vydání:	2018
Předmět:	Male Accuracy and precision Pharmaceutical Science 01 natural sciences Piperazines Article Analytical Chemistry lcsh:QD241-441 Rats Sprague-Dawley 03 medical and health sciences T790M 0302 clinical medicine lcsh:Organic chemistry Pharmacokinetics Tandem Mass Spectrometry Epidermal growth factor Drug Discovery Animals Protein precipitation rat Osimertinib UPLC-TOF-MS Physical and Theoretical Chemistry Chromatography High Pressure Liquid Acrylamides Aniline Compounds Chromatography Molecular Structure Chemistry 010401 analytical chemistry Organic Chemistry Extraction (chemistry) Reproducibility of Results Rats 0104 chemical sciences Chemistry (miscellaneous) osimertinib 030220 oncology & carcinogenesis Molecular Medicine Time-of-flight mass spectrometry pharmacokinetics
Zdroj:	Molecules Volume 23 Issue 11 Molecules, Vol 23, Iss 11, p 2894 (2018)
ISSN:	1420-3049
DOI:	10.3390/molecules23112894
Popis:	Osimertinib, a new-generation inhibitor of the epidermal growth factor, has been used for the clinical treatment of advanced T790M mutation-positive tumors. In this research, an original analysis method was established for the quantification of osimertinib by ultra-performance liquid chromatography with time of flight mass spectrometry (UPLC-TOF-MS) in rat plasma. After protein precipitation with acetonitrile and sorafinib (internal standard, IS), they were chromatographed through a Waters XTerra MS C18 column. The mobile phase was acetonitrile and water (including 0.1% ammonia). The relative standard deviation (RSD) of the intra- and inter-day results ranged from 5.38 to 9.76% and from 6.02 to 9.46%, respectively, and the extraction recovery and matrix effects were calculated to range from 84.31 to 96.14% and from 91.46 to 97.18%, respectively. The results illustrated that the analysis method had sufficient specificity, accuracy and precision. Meanwhile, the UPLC-TOF-MS method for osimertinib was successfully applied into the pharmacokinetics of SD rats.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9123f2d68267ce17db7949f7a6665f51 https://doi.org/10.3390/molecules23112894 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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