Rapid mechanoenzymatic saccharification of lignocellulosic biomass without bulk water or chemical pre-treatment
| Autor: | Karine Auclair, Tomislav Friščić, Marie-Josée Dumont, Fabien Hammerer, Karolin Dietrich, Shaghayegh Ostadjoo, Luis F. Del Rio |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
2. Zero hunger
010405 organic chemistry Chemistry Biomass Lignocellulosic biomass food and beverages Raw material Straw 010402 general chemistry Pulp and paper industry Pollution 01 natural sciences 7. Clean energy 0104 chemical sciences Hydrolysis Corn stover Environmental Chemistry Bagasse Renewable resource |
| DOI: | 10.1101/2020.03.06.980631 |
| Popis: | Lignocellulosic material is an abundant renewable resource with the potential to replace petroleum as a feedstock for the production of fuels and chemicals. The large scale deployment of biomass saccharification is, however, hampered by the necessity to use aggressive reagents and conditions, formation of side-products, and the difficulty to reach elevated monosaccharide concentrations in the crude product. Herein we report the high efficacy of Reactive Aging (or Raging, a technique where enzymatic reaction mixtures, without any bulk aqueous or organic solvent, are treated to multiple cycles of milling and aging) for gram-scale saccharification of raw lignocellulosic biomass samples from different agricultural sources (corn stover, wheat straw, and sugarcane bagasse). The solvent-free enzymatic conversion of lignocellulosic biomass was found to proceed in excellent yields (ca. 90%) at protein loadings as low as 2% w/w, without the need for any prior chemical pre-treatment or high temperatures, to produce highly concentrated (molar) monosaccharides. This crude product of mechanoenzymatic depolymerization is non-toxic to bacteria and can be used as a carbon source for bacterial growth. |
| Databáze: | OpenAIRE |
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