Activation analysis of serum vitamin B-12
| Autor: | Carl Jansen, Manuel Navarrette, George Leddicotte |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
inorganic chemicals
Square Centimeter business.industry Radiochemistry chemistry.chemical_element Neutron temperature Activation Analysis Vitamin B 12 Blood serum chemistry Immunology Methods Medicine Radiology Nuclear Medicine and imaging Neutron Vitamin B12 Irradiation Neutron activation analysis business Cobalt |
| Zdroj: | Radiology. 91(4) |
| ISSN: | 0033-8419 |
| Popis: | The estimation of serum vitamin B12 is frequently required in the work-up of megaloblastic anemias. The procedure of choice—although it has not proved to be accurate—has been a microbiological assay (3) which takes from two to five days to complete. Neutron activation analysis (1) would offer a simple, rapid, and accurate method for determination of serum vitamin B12 levels. Vitamin B12 has a cobalt atom in the center of the molecule, and cobalt is not found elsewhere in the serum. Thus, the amount of cobalt is proportional to the amount of vitamin B12. By the use of the cobalt 60m(10.5 m) resulting from the thermal neutron reaction, 69Co(n,γ)60mCo, it is possible to measure the serum levels of vitamin B12. Procedure Using the University of Missouri research reactor, located at Columbia, one can achieve a sensitivity of 10-12 g per milliliter of vitamin B12 by irradiating a sample for three minutes in a thermal neutron flux of 5.0 X 1013 neutrons per square centimeter per second. After irradiation, the ac... |
| Databáze: | OpenAIRE |
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