Effects of succinylacetone on growth and respiration of L1210 leukemia cells
| Autor: | Paul S. Ebert, Eugene C. Weinbach |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Cancer Research
Oligomycin Cellular respiration Cell growth Oxidative phosphorylation Mitochondrion Biology Molecular biology Heptanoates Mice chemistry.chemical_compound Oxygen Consumption Oncology chemistry Biochemistry Heptanoic Acids Mice Inbred DBA Respiration Animals L1210 cells Leukemia L1210 Heme Cells Cultured |
| Zdroj: | Cancer Letters. 26:253-259 |
| ISSN: | 0304-3835 |
| Popis: | 4,6-Dioxoheptanoic acid (succinylacetone, SA), a potent inhibitor of heme biosynthesis, suppressed growth and decreased respiration of L1210 leukemia cells in vitro. Growth of cells incubated in the presence of 2–4 mM SA for the first 2 days declined, and after 3 days virtually ceased. L1210 cells in the logarithmic growth phase exhibited active respiration (40 ± 9.3 nanoatoms oxygen/min · 107 cells at 37°C) which was inhibited by oligomycin and released by uncouplers of oxidative phosphorylation. These and other inhibitors of mitochondrial function clearly demonstrate a mitochondrial basis for the cellular respiration in both control and SA-treated cells. L1210 cell in the stationary phase exhibited a marked decrease in oxygen consumption compared to cells in logarithmic growth. At the concentrations used in this study, SA was not immediately toxic to L1210 cells, but inhibited growth at 2 days without lowering levels of cellular heme. Thus, it appears unlikely that inhibition of growth of L1210 cells by SA can be ascribed either to heme depletion or to impairment of respiration. |
| Databáze: | OpenAIRE |
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