Acute effect of androgens on maximal force-generating capacity and electrically evoked calcium transient in mouse skeletal muscles
| Autor: | Gillian Butler-Browne, Daniel Metzger, Arnaud Ferry, Alban Vignaud, Bourama Fane, Mélanie Schuh, Bodvael Fraysse |
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| Přispěvatelé: | Service d'Oto-Rhino-Laryngologie et d'otoneurochirurgie, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse], Immunologie moléculaire et biothérapies innovantes (IMBI), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Généthon, UMRS974, Université Pierre et Marie Curie - Paris 6 (UPMC), U974, Institut National de la Santé et de la Recherche Médicale (INSERM), UMR7215, Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), École Pratique des Hautes Études (EPHE), Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Electric Stimulation medicine.drug_class [SDV]Life Sciences [q-bio] Clinical Biochemistry Muscle Contraction/*drug effects chemistry.chemical_element Stimulation Hindlimb Calcium Biochemistry Contractility Mice 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine Receptors Calcium Signaling/*drug effects medicine Animals Myocyte Calcium Signaling Muscle Skeletal Molecular Biology Skeletal/*cytology/drug effects/metabolism/*physiology 030304 developmental biology Pharmacology 0303 health sciences Organic Chemistry Skeletal muscle Androgen Androgen receptor Androgen/deficiency medicine.anatomical_structure chemistry Receptors Androgen Androgens Muscle Androgens/*pharmacology 030217 neurology & neurosurgery Muscle Contraction |
| Zdroj: | Steroids Steroids, 2014, 87, pp.6-11. ⟨10.1016/j.steroids.2014.05.005⟩ |
| ISSN: | 0039-128X |
| DOI: | 10.1016/j.steroids.2014.05.005 |
| Popis: | International audience; As androgens might have rapid androgen-receptor (AR) independent action on muscle cells, we analysed the in vivo acute effect of androgens on maximal force generation capacity and electrically evoked calcium transient responsible for the excitation-contraction coupling in skeletal muscle from wild-type male mice and muscle fibre androgen receptor (AR) deficient (AR(skm-/y)) male mice. We tested the hypothesis that acute in vivo androgen treatment improves contractility and modifies calcium transient in mouse hindlimb muscles. In addition, we determined whether the reduced maximal force generation capacity of AR(skm-/y) mice is caused by an alteration in calcium transient. We found that acute dehydrotestosterone (DHT) and testosterone treatment of mice does not change in situ maximal force, power or fatigue resistance of tibialis anterior muscles. In agreement with this observation, maximal force and twitch kinetics also remained unchanged when both whole extensor digitorum longus (EDL) muscle or fibre bundles were incubated in vitro with DHT. Electrically evoked calcium transient, i.e. calcium amplitude, time to peak and decay, was also not modified by DHT treatment of EDL muscle fibre bundles. Finally, we found no difference in calcium transient between AR(skm-/y) and wild-type mice despite the reduced maximal force in EDL fibre bundles of AR(skm-/y) mice. In conclusion, acute androgen treatment has no ergogenic effect on muscle contractility and does not affect calcium transient in response to stimulation. In addition, the reduced maximal force of AR(skm-/y) mice is not related to calcium transient dysfunction. |
| Databáze: | OpenAIRE |
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