EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes
| Autor: | Noritaka Saeki, Kazushige Ogawa, Midori Mukai, Norihiko Suruga |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Integrins Cellular differentiation Integrin Bone Marrow Cells HL-60 Cells Biology Ligands Monocytes 03 medical and health sciences Mice medicine Animals Humans lcsh:QH573-671 Cell adhesion EphA Cells Cultured Receptors Eph Family Cell adhesion molecule lcsh:Cytology Monocyte Erythropoietin-producing hepatocellular (Eph) receptor Cell Differentiation Ephrin-A1 Cell Biology Cell biology Up-Regulation 030104 developmental biology medicine.anatomical_structure HL60 Monocyte differentiation Differentiation biology.protein Cell Surface Extensions Signal transduction Ephrin-A Ephrins Research Article Signal Transduction |
| Zdroj: | BMC Cell Biology BMC Cell Biology, Vol 18, Iss 1, Pp 1-20 (2017) |
| ISSN: | 1471-2121 |
| Popis: | Background Eph signaling is known to induce contrasting cell behaviors such as promoting and inhibiting cell adhesion/spreading by altering F-actin organization and influencing integrin activities. We have previously demonstrated that EphA2 stimulation by ephrin-A1 promotes cell adhesion through interaction with integrins and integrin ligands in two monocyte/macrophage cell lines. Although mature mononuclear leukocytes express several members of the EphA/ephrin-A subclass, their expression has not been examined in monocytes undergoing during differentiation and maturation. Results Using RT-PCR, we have shown that EphA2, ephrin-A1, and ephrin-A2 expression was upregulated in murine bone marrow mononuclear cells during monocyte maturation. Moreover, EphA2 and EphA4 expression was induced, and ephrin-A4 expression was upregulated, in a human promyelocytic leukemia cell line, HL60, along with monocyte differentiation toward the classical CD14++CD16− monocyte subset. Using RT-PCR and flow cytometry, we have also shown that expression levels of αL, αM, αX, and β2 integrin subunits were upregulated in HL60 cells along with monocyte differentiation while those of α4, α5, α6, and β1 subunits were unchanged. Using a cell attachment stripe assay, we have shown that stimulation by EphA as well as ephrin-A, likely promoted adhesion to an integrin ligand-coated surface in HL60 monocytes. Moreover, EphA and ephrin-A stimulation likely promoted the formation of protrusions in HL60 monocytes. Conclusions Notably, this study is the first analysis of EphA/ephrin-A expression during monocytic differentiation/maturation and of ephrin-A stimulation affecting monocyte adhesion to an integrin ligand-coated surface. Thus, we propose that monocyte adhesion via integrin activation and the formation of protrusions is likely promoted by stimulation of EphA as well as of ephrin-A. Electronic supplementary material The online version of this article (doi:10.1186/s12860-017-0144-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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