Non-Covalently Stabilized Alginate Hydrogels as Functional Cell Scaffold Material

Autor: Mathijs van Poll, Swen Groen, Roelof Kuijer, Joris van Aken, Theo G. van Kooten, Philipp T. Kühn, Thomas L. Meijer, Patrick van Rijn, Irene Schiavon
Přispěvatelé: Polymer Chemistry and Bioengineering, Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), Nanotechnology and Biophysics in Medicine (NANOBIOMED)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Polymers and Plastics
cell scaffold
02 engineering and technology
01 natural sciences
Gelatin
BIOCOMPATIBILITY
chemistry.chemical_compound
Glucuronic Acid
DESIGN
CHEMISTRY
Materials Testing
Materials Chemistry
Polyethyleneimine
PEPTIDE
Cell encapsulation
Tissue Scaffolds
Hexuronic Acids
Hydrogels
MECHANICAL-PROPERTIES
021001 nanoscience & nanotechnology
Controlled release
Polyelectrolyte
Covalent bond
Self-healing hydrogels
0210 nano-technology
REGENERATIVE MEDICINE
Biotechnology
food.ingredient
Biocompatibility
Alginates
PHYSICAL-PROPERTIES
Bioengineering
Nanotechnology
Bone Marrow Cells
010402 general chemistry
Cell Line
food
BIOMEDICAL APPLICATIONS
Humans
BIOMATERIALS
polyelectrolyte
GELATIN
Mesenchymal Stem Cells
Fibroblasts
Glucuronic acid
0104 chemical sciences
chemistry
encapsulation
hydrogel
controlled release
Bandages
Hydrocolloid
Zdroj: Macromolecular Bioscience, 16(11), 1693-1702. WILEY-V C H VERLAG GMBH
ISSN: 1616-5187
Popis: Biopolymers are an attractive class of compounds for being used in biomedical applications as they are widely available from biomass. Their drawback is the lack of mechanical stability and the ability to tune this properly. Covalent chemical cross-linking is an often used approach but it limits usability due to legislation as well as the need of advanced and specialized knowledge by end users such as clinicians. Here, increased and tunable mechanical properties are achieved of alginate-based hydrogels with non-covalent approaches using linear polyethyleneimine (LPEI) as a polyelectrolyte rather than only multivalent metal ions (Ca2+). Gel stiffness increases with increasing LPEI content. Gel morphology changes from a thin fibrous mesh for alginate-Ca2+ to thicker fibrous networks when LPEI is introduced. The gels are able to efficiently release encapsulated small molecular dyes and the gels are able to host cells. For the cell encapsulation human skin fibroblasts (HSkF) and human bone marrow-derived mesenchymal stem cells (hBM-MSC) are used. HSkF can be successfully incorporated without diminished viability while the matrix components and gel preparation method are not compatible with hBM-MSC. The newly developed alginate-based system is regarded as a potential candidate for wound dressing materials.
Databáze: OpenAIRE
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