Old dog, new tricks: Influenza A virus NS1 and in vitro fibrillogenesis
| Autor: | Mariia Sergeeva, Irina L. Baranovskaya, Sergey M. Shishlyannikov, I.I. Savin, Edward Ramsay, A. N. Gorshkov, Vladimir V. Egorov, Aram A. Shaldzhyan, A.P. Kukhareva, Yana A. Zabrodskaya, A.V. Protasov |
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| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular Cell signaling Amyloid viruses Proteolysis medicine.medical_treatment Protein aggregation Viral Nonstructural Proteins medicine.disease_cause Fibril Microscopy Atomic Force Biochemistry Protein Aggregates Immune system medicine Influenza A virus Protease medicine.diagnostic_test Chemistry virus diseases Fibrillogenesis Congo Red General Medicine biochemical phenomena metabolism and nutrition Recombinant Proteins Cell biology Kinetics Microscopy Electron |
| Zdroj: | Biochimie. 190 |
| ISSN: | 1638-6183 |
| Popis: | The influenza NS1 protein is involved in suppression of the host immune response. Recently, there is growing evidence that prion-like protein aggregation plays an important role in cellular signaling and immune responses. In this work, we obtained a recombinant, influenza A NS1 protein and showed that it is able to form amyloid-like fibrils in vitro. Using proteolysis and subsequent mass spectrometry, we showed that regions resistant to protease hydrolysis highly differ between the native NS1 form (NS1-N) and fibrillar form (NS1-F); this indicates that significant structural changes occur during fibril formation. We also found a protein fragment that is capable of inducing the process of fibrillogenesis at 37 °C. The discovery of the ability of NS1 to form amyloid-like fibrils may be relevant to uncovering relationships between influenza A infection and modulation of the immune response. |
| Databáze: | OpenAIRE |
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