A rapid method for the determination of guanosine 5'-diphosphate-3'-diphosphate and guanosine 5'-triphosphate-3'-diphosphate by high-performance liquid chromatography
| Autor: | Steven A. Short, Thomas P. Zimmerman, Meir Fischer |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Formic acid
Biophysics Guanosine Guanosine Tetraphosphate Biochemistry High-performance liquid chromatography chemistry.chemical_compound Mice Potassium phosphate Guanosine 5'-Diphosphate Escherichia coli Animals Humans Nucleotide Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Elution Guanosine Pentaphosphate Magnesium acetate Cell Biology Neoplasms Experimental Guanine Nucleotides Mice Inbred C57BL chemistry |
| Zdroj: | Analytical biochemistry. 121(1) |
| ISSN: | 0003-2697 |
| Popis: | A method is described for the rapid analysis of the nucleotides, guanosine 5′-diphosphate-3′-diphosphate (ppGpp) and guanosine 5′-triphosphate-3′-diphosphate (pppGpp), by high-performance liquid chromatography. It has been found that the inclusion of magnesium acetate in the potassium phosphate buffer facilitates elution of these highly phosphorylated compounds from the Partisil strong anion-exchange resin and allows their determination under isocratic conditions. The application of this methodology to formic acid extracts of bacterial cells is demonstrated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|