Identification of a candidate stem cell in human gallbladder
| Autor: | William A. LaFramboise, Lynda Guzik, Rohan Manohar, Helene Fohrer, Yaming Li, Donna B. Stolz, Eric Lagasse, Uma R. Chandran |
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| Přispěvatelé: | University of Pittsburgh School of Medicine, Pennsylvania Commonwealth System of Higher Education (PCSHE), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Pitié-Salpêtrière [APHP] |
| Rok vydání: | 2015 |
| Předmět: |
Cellular differentiation
[SDV]Life Sciences [q-bio] Cell [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity chemistry.chemical_compound 0302 clinical medicine Cell Self Renewal lcsh:QH301-705.5 Cells Cultured ComputingMilieux_MISCELLANEOUS Oligonucleotide Array Sequence Analysis Medicine(all) 0303 health sciences education.field_of_study biology Stem Cells Gallbladder Epithelial cell adhesion molecule Cell Differentiation General Medicine Epithelial Cell Adhesion Molecule 3. Good health medicine.anatomical_structure Hyaluronan Receptors Phenotype [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology 030220 oncology & carcinogenesis Stem cell Cell type Population [SDV.CAN]Life Sciences [q-bio]/Cancer CD13 Antigens Article 03 medical and health sciences Antigens Neoplasm medicine Humans education 030304 developmental biology Gene Expression Profiling CD44 Cell Biology Molecular biology Bile Ducts Intrahepatic chemistry lcsh:Biology (General) Immunology biology.protein Cell Adhesion Molecules Biomarkers Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 14, Iss 3, Pp 258-269 (2015) Stem Cell Research Stem Cell Research, Elsevier, 2015, 14 (3), pp.258-269. ⟨10.1016/j.scr.2014.12.003⟩ Stem cell research Stem cell research, Elsevier, 2015, 14 (3), pp.258-269. ⟨10.1016/j.scr.2014.12.003⟩ |
| ISSN: | 1873-5061 1876-7753 |
| DOI: | 10.1016/j.scr.2014.12.003 |
| Popis: | International audience; There are currently no reports of identification of stem cells in human gallbladder. The differences between human gallbladder and intrahepatic bile duct (IHBD) cells have also not been explored. The goals of this study were to evaluate if human fetal gallbladder contains a candidate stem cell population and if fetal gallbladder cells are distinct from fetal IHBD cells. We found that EpCAM+CD44+CD13+ cells represent the cell population most enriched for clonal self-renewal from primary gallbladder. Primary EpCAM+CD44+CD13+ cells gave rise to EpCAM+CD44+CD13+ and EpCAM+CD44+CD13- cells in vitro, and gallbladder cells expanded in vitro exhibited short-term engraftment in vivo. Last, we found that CD13, CD227, CD66, CD26 and CD49b were differentially expressed between gallbladder and IHBD cells cultured in vitro indicating clear phenotypic differences between the two cell populations. Microarray analyses of expanded cultures confirmed that both cell types have unique transcriptional profiles with predicted functional differences in lipid, carbohydrate, nucleic acid and drug metabolism. In conclusion, we have isolated a distinct clonogenic population of epithelial cells from primary human fetal gallbladder with stem cell characteristics and found it to be unique compared to IHBD cells. |
| Databáze: | OpenAIRE |
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