In vitro and in vivo experimental hepatotoxic models in liver research: applications to the assessment of potential hepatoprotective drugs
| Autor: | M Kgalalelo Kemelo, L Wojnarová, N Kutinová Canová, H Farghali |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Biomedical Research Physiology Down-Regulation Galactosamine Resveratrol Pharmacology Protective Agents 03 medical and health sciences chemistry.chemical_compound Liver disease 0302 clinical medicine Pharmacotherapy Sirtuin 1 In vivo Drug Discovery Stilbenes medicine Animals Humans Hepatitis biology Chemistry General Medicine Liver Failure Acute medicine.disease In vitro Disease Models Animal 030104 developmental biology Hepatoprotection 030220 oncology & carcinogenesis biology.protein |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1802-9973 |
| Popis: | This mini-review highlights our and others’ experience about in vitro and in vivo models that are being used to follow up events of liver injuries under various hepatotoxic agents and potential hepatoprotective drugs. Due to limitations of the outcomes in each model, we focus primarily on two models. First, a developed perfusion method for isolated immobilized hepatocytes that improves the process of oxygenation and helps in end-product removal is of considerable value in improving cell maintenance. This cellular model is presented as a short-term research-scale laboratory bioreactor with various physiological, biochemical, molecular, toxicological and pharmacological applications. Second, the in vivo model of D-galactosamine and lipopolysaccharide (D-GalN/LPS) combination-induced liver damage is described with some details. Recently, we have revealed that resveratrol and other natural polyphenols attenuate D-GalN/LPS-induced hepatitis. Moreover, we reported that D-GalN/LPS down-regulates sirtuin 1 in rat liver. Therefore, we discuss here the role of sirtuin 1 modulation in hepatoprotection. A successful development of pharmacotherapy for liver diseases depends on the suitability of in vitro and in vivo hepatic injury systems. Several models are available to screen the hepatotoxic or hepatoprotective activity of any substance. It is important to combine different methods for confirmation of the findings. |
| Databáze: | OpenAIRE |
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