Effect of radicicol infusion on the Src tyrosine kinase activity of rat hippocampus before and after training in an inhibitory avoidance task
| Autor: | Carlos Alberto Gonçalves, Patrícia Pereira, Lucimar Filot da Silva Brum, Ivan Izquierdo, Letícia Rodrigues, Patrícia Ardenghi, Elsa Regina do Canto Vinade, Tiago Luis Dedavid e Silva |
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| Rok vydání: | 2004 |
| Předmět: |
Male
animal structures Hippocampus Hippocampal formation Pharmacology Biochemistry Cellular and Molecular Neuroscience chemistry.chemical_compound Memory Avoidance Learning Animals Enzyme Inhibitors Rats Wistar Kinase General Medicine Impaired memory Radicicol Rats src-Family Kinases chemistry Memory consolidation Macrolides Psychology Tyrosine kinase Neuroscience Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Neurochemical research. 32(7) |
| ISSN: | 0364-3190 |
| Popis: | The participation of protein serine/threonine kinases in memory formation and retrieval is well established. In contrast, relatively little is known on the role of protein tyrosine kinases (PTKs). Previous work showed that intra-hippocampal infusion of the Src-PTK inhibitor radicicol inhibits memory acquisition, consolidation, and retrieval of one-trial step-down inhibitory avoidance task. In this study, we investigated the possible interaction between levels of Src-PTK activity in hippocampus and memory acquisition, formation, and retrieval of this task. Radicicol (0.5 microg/ml) was infused into the CA1 region of the hippocampus of rats trained in a one-trial step-down inhibitory avoidance task. Radicicol infused 15 min before training decreased Src-PTK activity, as measured 0, 1.5, and 24 h after training, and impaired memory acquisition of the task. When given immediately after training, there was a decrease in Src-PTK activity 1.5 h, but not 0 or 24 h after training. This treatment depressed memory consolidation. Radicicol infused into CA1 10 min prior to retrieval testing inhibited hippocampal Src-PTK activity, as measured immediately after the test session. The results suggest that Src-PTKs participate in memory acquisition, consolidation, and retrieval processes, but the timing of the role of the enzyme is different in each case. |
| Databáze: | OpenAIRE |
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