The Application of a Commercially Available Citrus-Based Extract Mitigates Moderate NaCl-Stress in Arabidopsis thaliana Plants
| Autor: | Johannes Loubser, P.N. Hills |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine malondialdehyde BC204 Arabidopsis Plant Science SOS1 01 natural sciences Article anthocyanin salinity 03 medical and health sciences chemistry.chemical_compound lcsh:Botany Arabidopsis thaliana Proline proline Ecology Evolution Behavior and Systematics Ecology biology fungi food and beverages biology.organism_classification Malondialdehyde lcsh:QK1-989 Salinity Horticulture biostimulants 030104 developmental biology chemistry Chlorophyll Anthocyanin Shoot 010606 plant biology & botany |
| Zdroj: | Plants Volume 9 Issue 8 Plants, Vol 9, Iss 1010, p 1010 (2020) |
| ISSN: | 2223-7747 |
| Popis: | Aims: The aim of this study was to assess the effect of BC204 as a plant biostimulant on Arabidopsis thaliana plants under normal and NaCl-stressed conditions. Methods: For this study, ex vitro and in vitro growth experiments were conducted to assess the effect of both NaCl and BC204 on basic physiological parameters such as biomass, chlorophyll, proline, malondialdehyde, stomatal conductivity, Fv/Fm and the expression of four NaCl-responsive genes. Results: This study provides preliminary evidence that BC204 mitigates salt stress in Arabidopsis thaliana. BC204 treatment increased chlorophyll content, fresh and dry weights, whilst reducing proline, anthocyanin and malondialdehyde content in the presence of 10 dS· m&minus 1 electroconductivity (EC) salt stress. Stomatal conductivity was also reduced by BC204 and NaCl in source leaves. In addition, BC204 had a significant effect on the expression of salinity-related genes, stimulating the expression of salinity-related genes RD29A and SOS1 independently of NaCl-stress. Conclusions: BC204 stimulated plant growth under normal growth conditions by increasing above-ground shoot tissue and root and shoot growth in vitro. BC204 also increased chlorophyll content while reducing stomatal conductivity. BC204 furthermore mitigated moderate to severe salt stress (10&ndash 20 dS· 1) in A. thaliana. Under salt stress conditions, BC204 reduced the levels of proline, anthocyanin and malondialdehyde. The exact mechanism by which this occurs is unknown, but the results in this study suggest that BC204 may act as a priming agent, stimulating the expression of genes such as SOS1 and RD29A. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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