Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles
| Autor: | Jose L. Lopez-Ribot, Humberto H Lara, M. Josefina Arellano-Jiménez, Dulce G. Romero-Urbina, Miguel Jose Yacaman, J. Jesús Velázquez-Salazar, Anand Srinivasan, Eduardo Larios |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
silver nanoparticles
Staphylococcus aureus Lysis General Physics and Astronomy Biology lcsh:Chemical technology medicine.disease_cause lcsh:Technology Silver nanoparticle Bacterial cell structure Full Research Paper Microbiology wall teichoic acids Cell wall chemistry.chemical_compound medicine Nanotechnology lcsh:TP1-1185 General Materials Science Electrical and Electronic Engineering lcsh:Science Teichoic acid electron microscopy methicillin-resistant Staphylococcus aureus (MRSA) lcsh:T lcsh:QC1-999 positively charged nanoparticles Nanoscience chemistry Biophysics lcsh:Q Peptidoglycan lcsh:Physics Intracellular |
| Zdroj: | Beilstein Journal of Nanotechnology Beilstein Journal of Nanotechnology, Vol 6, Iss 1, Pp 2396-2405 (2015) |
| ISSN: | 2190-4286 |
| Popis: | Silver nanoparticles offer a possible means of fighting antibacterial resistance. Most of their antibacterial properties are attributed to their silver ions. In the present work, we study the actions of positively charged silver nanoparticles against both methicillin-sensitiveStaphylococcus aureusand methicillin-resistantStaphylococcus aureus. We use aberration-corrected transmission electron microscopy to examine the bactericidal effects of silver nanoparticles and the ultrastructural changes in bacteria that are induced by silver nanoparticles. The study revealed that our 1 nm average size silver nanoparticles induced thinning and permeabilization of the cell wall, destabilization of the peptidoglycan layer, and subsequent leakage of intracellular content, causing bacterial cell lysis. We hypothesize that positively charged silver nanoparticles bind to the negatively charged polyanionic backbones of teichoic acids and the related cell wall glycopolymers of bacteria as a first target, consequently stressing the structure and permeability of the cell wall. This hypothesis provides a major mechanism to explain the antibacterial effects of silver nanoparticles onStaphylococcus aureus.Future research should focus on defining the related molecular mechanisms and their importance to the antimicrobial activity of silver nanoparticles. |
| Databáze: | OpenAIRE |
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