The presence of two S-layer-protein-encoding genes is conserved among species related to Lactobacillus acidophilus
| Autor: | Carin P. A. M. Kolen, Hein J. Boot, Karel Kersters, Bruno Pot, Peter H. Pouwels |
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| Přispěvatelé: | Prins Maurits Laboratorium TNO, Molecular Microbial Physiology (SILS, FNWI), Department of Bio-engineering Sciences |
| Rok vydání: | 1996 |
| Předmět: |
Surface layer
Homology (biology) Bacterial protein Lactobacillus acidophilus Lactobacillus gasseri Polyacrylamide gel electrophoresis Lactobacillus crispatus Bacteria (microorganisms) Priority journal Genetics SDS-PAGE identification Membrane Proteins/analysis Lactobacillus gallinarum Lactobacillus acidophilus/genetics biology Bacterial gene Southern blotting Chromosome Mapping food and beverages Blotting Southern Electrophoresis Polyacrylamide Gel Genetic conservation S-layer Bacterial Proteins/analysis Chromosome inversion Immunoblotting Blotting Western Lactobacillus delbrueckii subsp. bulgaricus S-layer variation Microbiology Bacterial Proteins Sequence Homology Nucleic Acid Biology Gene Lactobacillus johnsonii Southern blot Sequence Homology Amino Acid Lactobacillus amylovorus Membrane Proteins Nonhuman equipment and supplies biology.organism_classification S-protein Molecular biology Lactobacillus helveticus Lactobacillus Silent gene |
| Zdroj: | Microbiology, 142, 2375-2384. Maik Nauka-Interperiodica Publishing Microbiology, 9, 142, 2375-2384 |
| ISSN: | 1465-2080 1350-0872 0026-2617 |
| DOI: | 10.1099/00221287-142-9-2375 |
| Popis: | Previously we have shown that the type strain of Lactobacillus acidophilus possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The S-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of this slp segment. In this study the presence of S-protein and corresponding S-protein-encoding genes of strains belonging to species that are closely related to L. acidophilus was determined. All strains investigated were identified by numerical comparison of highly standardized one-dimensional SDS-PAGE whole-cellular-protein patterns. Western blot and Southern blot methods were used to identify the presence of, and homology between, S-proteins and S-protein-encoding genes. From these analyses we conclude that strains of L. acidophilus, L. crispatus, L. amylovorus and L. gallinarum possess an S-layer and contain two slp genes. Strains of L. helveticus possess an S-layer but have only one intact slp gene. Strains of L. gasseri, L. johnsonii and L. delbrueckii subsp. bulgaricus have neither an S-layer nor S-protein-encoding genes hybridizing with probes derived from the L. acidophilus slpA or slpB region. The presence of a highly conserved 5' region in the slp genes of strains of L. acidophilus, L. crispatus, L. amylovorus and L. gallinarum suggests that S-layer variation is a common feature for strains of these species. |
| Databáze: | OpenAIRE |
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