[Perfil de resistência do Rhipicephalus (Boophilus) microplus ao diazinon e à cipermetrina e primeiro relato de mutação no canal de sódio - domain III S6 - T2134A em amostras de campo, no estado de São Paulo - Brasil]

Autor: M.L. Santos, L.C. Fiorini, F.C. Duarte, K.A. Anjos, A.F.C. Nassar, L.G. Brito, J.R. Pereira, M.C. Mendes
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.74 n.3 2022
Arquivo brasileiro de medicina veterinária e zootecnia
Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Volume: 74, Issue: 3, Pages: 473-482, Published: 10 JUN 2022
Popis: Rhipicephalus (Boophilus) microplus is one of the parasites that has an impact on livestock farming in Brazil. Bioassays using the larval packet test (LPT) impregnated with cypermethrin and diazinon at different concentrations were performed to characterize phenotypic resistance (resistance level, RL), molecular resistance (mutation in domain III - S6 T2134A) and enzymatic metabolism of diazinon and cypermethrin in some populations of R. microplus that were collected in different regions of the state of São Paulo. Among the 40 farms analyzed, 18 of them presented resistance factors for cypermethrin, with RL I (53%) and RL II (47%). Mutation of domain III T2134A was found for the first time in Brazil, on seven farms. Heterozygous larvae were found on six of them and resistant homozygous larvae on four. No differences (p>0.05) in enzymatic activity of α-esterase and β-esterase were found in tests with live and dead larvae at a cypermethrin concentration of 409.6μg/cm2. Ninety percent of the farms showed resistance to the active agent diazinon: RL I in 6%, RL II in 30% and RL III in 64%. There were significant differences (p0,05) na atividade enzimática de α-esterase e β-esterase foi observada em testes com larvas vivas e mortas em uma concentração de cipermetrina de 409,6μg/cm2. Noventa por cento das propriedades apresentaram resistência ao agente ativo diazinon: RL I em 6%, RL II em 30% e RL III em 64%. Houve diferenças significativas (P
Databáze: OpenAIRE
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