Prednisolone induces the Wnt signalling pathway in 3T3-L1 adipocytes
| Autor: | Wim H. A. Dokter, Bruno Guigas, Jacob de Vlieg, Wynand Alkema, Margot M. Linssen, Wilco W. M. Fleuren, D. Margriet Ouwens, Erik J M Toonen, Gerard C.M. van der Zon |
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| Přispěvatelé: | Data Sciences for Life Science & Health |
| Rok vydání: | 2013 |
| Předmět: |
Physiology
medicine.medical_treatment Gene Expression Adipose tissue transcriptome/drug effects prednisolone/adverse effects adipocytes/drug effects 0302 clinical medicine insulin resistance Adipocytes Insulin Wnt Signaling Pathway 0303 health sciences glucocorticoids adipokines/genetics insulin/pharmacology Health aging / healthy living Pathogenesis and modulation of inflammation [IGMD 5] Wnt signaling pathway signal transduction/drug effects General Medicine 3. Good health Cell biology immunity/drug effects 3t3-l1 cells Signal transduction Signal Transduction Chemical and physical biology [NCMLS 7] medicine.medical_specialty mice Prednisolone Adipokine 030209 endocrinology & metabolism Deoxyglucose Biology glucocorticoids/adverse effects 03 medical and health sciences Insulin resistance Adipokines Gene profiling Physiology (medical) Internal medicine medicine Animals Secretion deoxyglucose/metabolism 030304 developmental biology metabolic dysfunction biochemie gene expression/drug effects Immunity 3T3-L1 Original Articles medicine.disease Endocrinology Tissue Array Analysis Research Programm of Institute for Molecules and Materials Transcriptome |
| Zdroj: | Archives of Physiology and Biochemistry, 119, 52-64 Archives of Physiology and Biochemistry, 119(2), 52-64 Archives of Physiology and Biochemistry Archives of Physiology and Biochemistry, 119(2), 52-64. Swets & Zeitlinger Archives of Physiology and Biochemistry, 119, 2, pp. 52-64 |
| ISSN: | 1381-3455 |
| Popis: | Contains fulltext : 117166.pdf (Publisher’s version ) (Open Access) Synthetic glucocorticoids are potent anti-inflammatory drugs but show dose-dependent metabolic side effects such as the development of insulin resistance and obesity. The precise mechanisms involved in these glucocorticoid-induced side effects, and especially the participation of adipose tissue in this are not completely understood. We used a combination of transcriptomics, antibody arrays and bioinformatics approaches to characterize prednisolone-induced alterations in gene expression and adipokine secretion, which could underlie metabolic dysfunction in 3T3-L1 adipocytes. Several pathways, including cytokine signalling, Akt signalling, and Wnt signalling were found to be regulated at multiple levels, showing that these processes are targeted by prednisolone. These results suggest that mechanisms by which prednisolone induce insulin resistance include dysregulation of wnt signalling and immune response processes. These pathways may provide interesting targets for the development of improved glucocorticoids. |
| Databáze: | OpenAIRE |
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