Schisandrin A inhibits dengue viral replication via upregulating antiviral interferon responses through STAT signaling pathway
| Autor: | Chin-Kai Tseng, Yu-Hsuan Wu, Jin-Ching Lee, Yen-Hsu Chen, Yao-Chin Hsu, Chun-Kuang Lin, Jung-Sheng Yu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Serotype medicine.drug_class viruses Dengue virus Virus Replication medicine.disease_cause Antiviral Agents Article Lignans Cell Line Dengue fever Dengue Cyclooctanes Mice 03 medical and health sciences Cricetulus Interferon In vivo Cell Line Tumor Cricetinae medicine Animals Humans Polycyclic Compounds STAT1 Mice Inbred ICR Multidisciplinary 030102 biochemistry & molecular biology biology business.industry virus diseases Dengue Virus biochemical phenomena metabolism and nutrition medicine.disease Virology Up-Regulation STAT Transcription Factors 030104 developmental biology Viral replication biology.protein Interferons Antiviral drug business Signal Transduction medicine.drug |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Dengue virus (DENV) infects 400 million people worldwide annually. Infection of more than one serotype of DENV highly corresponds to dengue hemorrhagic fever and dengue shock syndrome, which are the leading causes of high mortality. Due to lack of effective vaccines and unavailable therapies against DENV, discovery of anti-DENV agents is urgently needed. We first characterize that Schisandrin A can inhibit the replication of four serotypes of DENV in a concentration- and time-dependent manner, with an effective half-maximal effective concentration 50% (EC50) value of 28.1 ± 0.42 μM against DENV serotype type 2 without significant cytotoxicity. Furthermore, schisandrin A can effectively protect mice from DENV infection by reducing disease symptoms and mortality of DENV-infected mice. We demonstrate that STAT1/2-mediated antiviral interferon responses contribute to the action of schisandrin A against DENV replication. Schisandrin A represents a potential antiviral agent to block DENV replication in vitro and in vivo. In conclusion, stimulation of STAT1/2-mediated antiviral interferon responses is a promising strategy to develop antiviral drug. |
| Databáze: | OpenAIRE |
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