Highlighting Curcumin-Induced Crosstalk between Autophagy and Apoptosis as Supported by Its Specific Subcellular Localization
| Autor: | Franck Sureau, Nathan E. Rainey, Patrice X. Petit, José-Enrique O'Connor, Ana Saric, Francisco J. Sala de Oyanguren, Aoula Moustapha |
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| Přispěvatelé: | Sureau, Franck, Universitat de València (UV), Saints-Pères Paris Institute for Neurosciences (SPPIN - UMR 8003), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Sorbonne Université (SU), Laboratoire Jean Perrin (LJP), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Saints-Pères Paris Institute for Neurosciences (SPINN - UMR 8003), Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Université Sorbonne Paris Nord, Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Programmed cell death
autophagy Cell Membrane Permeability Curcumin [SDV]Life Sciences [q-bio] [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lysosome Cell Line Tumor xCELLigence medicine [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Humans cancer lcsh:QH301-705.5 030304 developmental biology real-time cellular impedance Cell Nucleus 0303 health sciences calcium Endoplasmic reticulum Autophagy apoptosis ROS General Medicine 3. Good health Cell biology Mitochondria endoplasmic reticulum medicine.anatomical_structure cell death chemistry lcsh:Biology (General) Apoptosis 030220 oncology & carcinogenesis Cancer cell Unfolded protein response Unfolded Protein Response lysosome Lysosomes [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Subcellular Fractions |
| Zdroj: | Cells Cells, 2020, 9, ⟨10.3390/cells9020361⟩ Cells 2020 Cells 2020, 2020, 9 (2), pp.361. ⟨10.3390/cells9020361⟩ Cells, MDPI, 2020, 9, ⟨10.3390/cells9020361⟩ r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) Centro de Investigación Principe Felipe (CIPF) Volume 9 Issue 2 r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) instname Cells, Vol 9, Iss 2, p 361 (2020) |
| ISSN: | 2073-4409 |
| Popis: | Curcumin, a major active component of turmeric (Curcuma longa, L.), is known to have various effects on both healthy and cancerous tissues. In vitro studies suggest that curcumin inhibits cancer cell growth by activating apoptosis, but the mechanism underlying the anticancer effect of curcumin is still unclear. Since there is a recent consensus about endoplasmic reticulum (ER) stress being involved in the cytotoxicity of natural compounds, we have investigated using Image flow cytometry the mechanistic aspects of curcumin&rsquo s destabilization of the ER, but also the status of the lysosomal compartment. Curcumin induces ER stress, thereby causing an unfolded protein response and calcium release, which destabilizes the mitochondrial compartment and induce apoptosis. These events are also associated with secondary lysosomal membrane permeabilization that occurs later together with an activation of caspase-8, mediated by cathepsins and calpains that ended in the disruption of mitochondrial homeostasis. These two pathways of different intensities and momentum converge towards an amplification of cell death. In the present study, curcumin-induced autophagy failed to rescue all cells that underwent type II cell death following initial autophagic processes. However, a small number of cells were rescued (successful autophagy) to give rise to a novel proliferation phase. |
| Databáze: | OpenAIRE |
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