In vivo monitoring and high-resolution characterizing of the prednisolone-induced osteoporotic process on adult zebrafish by optical coherence tomography
| Autor: | Deng Lijun, Chudan Gao, Yanping Lin, Xiang Xiang, Tingru Chen, Lvming Zeng, Jian Zhang, Hong-Bo Fu, Limei Wang |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
genetic structures medicine.diagnostic_test biology High resolution Magnetic resonance imaging biology.organism_classification Article eye diseases Atomic and Molecular Physics and Optics Optical imaging Three dimensional imaging Optical coherence tomography In vivo medicine sense organs Zebrafish Process (anatomy) Biotechnology Biomedical engineering |
| Zdroj: | Biomedical Optics Express. 10:1184 |
| ISSN: | 2156-7085 |
| DOI: | 10.1364/boe.10.001184 |
| Popis: | Because of its similar genetic makeup with humans, zebrafish are an available and well-established osteoporosis model in vivo for anti-osteoporosis drug development as well as the drug safety-evaluation process. However, few optical imaging methods could effectively visualize the bone of adult zebrafish due to their limited penetration depth. In this paper, in vivo high-resolution and long-term characterization of a prednisolone-induced osteoporotic zebrafish model was achieved with spectral-domain optical coherence tomography (SD-OCT). The capability of three-dimensional SD-OCT imaging was also demonstrated in this study. With SD-OCT images, we could non-destructively monitor the deforming process of adult zebrafish skull from several directions at any time. There is good correlation and agreement between SD-OCT and histology. Valuable phenomenon such as bone defects could be quantitatively evaluated using the SD-OCT images at different time points during a period of 21 days. |
| Databáze: | OpenAIRE |
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