Detection of respiratory syncytial virus defective genomes in nasal secretions is associated with distinct clinical outcomes
| Autor: | Kedir N. Turi, Allan Paras, Carolina B. López, Ana María Cárdenas, Agnieszka Jozwik, Kristen A. Feemster, Yan Sun, Sébastien A. Felt, Emna Achouri, Christopher Chiu, Larry J. Anderson, David C. Nickle, Maximillian S. Habibi, Shaon Sengupta, Tina V. Hartert, Meiping Chang |
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| Rok vydání: | 2021 |
| Předmět: |
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Microbiology (medical) viruses Immunology Mucous membrane of nose Genome Viral Respiratory Syncytial Virus Infections Applied Microbiology and Biotechnology Microbiology Genome Article Virus Defective virus Cohort Studies 03 medical and health sciences Disease severity 1108 Medical Microbiology Genetics Humans Medicine Respiratory system 030304 developmental biology 0303 health sciences Science & Technology 030306 microbiology business.industry Infant Newborn Defective Viruses Infant Cell Biology 3. Good health Nasal Mucosa Viral replication Respiratory Syncytial Virus Human Female business Life Sciences & Biomedicine Viral load 0605 Microbiology |
| Zdroj: | Nat Microbiol |
| ISSN: | 2058-5276 |
| Popis: | Respiratory syncytial virus (RSV) causes respiratory illness in children, immunosuppressed individuals and the elderly. However, the viral factors influencing the clinical outcome of RSV infections remain poorly defined. Defective viral genomes (DVGs) can suppress virus replication by competing for viral proteins and by stimulating antiviral immunity. We studied the association between detection of DVGs of the copy-back type and disease severity in three RSV A-confirmed cohorts. In hospitalized children, detection of DVGs in respiratory samples at or around the time of admission associated strongly with more severe disease, higher viral load and a stronger pro-inflammatory response. Interestingly, in experimentally infected adults, the presence of DVGs in respiratory secretions differentially associated with RSV disease severity depending on when DVGs were detected. Detection of DVGs early after infection associated with low viral loads and mild disease, whereas detection of DVGs late after infection, especially if DVGs were present for prolonged periods, associated with high viral loads and severe disease. Taken together, we demonstrate that the kinetics of DVG accumulation and duration could predict clinical outcome of RSV A infection in humans, and thus could be used as a prognostic tool to identify patients at risk of worse clinical disease. |
| Databáze: | OpenAIRE |
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