Identification and Characterization of an Animal Δ12 Fatty Acid Desaturase Gene by Heterologous Expression in Saccharomyces cerevisiae
| Autor: | Marlyse M. Peyou-Ndi, Jennifer L. Watts, John Browse |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Fatty Acid Desaturases
Membrane Fluidity Molecular Sequence Data Saccharomyces cerevisiae Biophysics Gene Expression Biology Biochemistry Complementary DNA Membrane fluidity Animals Histidine Amino Acid Sequence Cloning Molecular Caenorhabditis elegans Molecular Biology Genes Helminth chemistry.chemical_classification Ethanol Sequence Homology Amino Acid Cell Membrane Temperature Hydrogen Peroxide Physical Chromosome Mapping biology.organism_classification Yeast Kinetics Oxidative Stress Fatty acid desaturase chemistry Fatty Acids Unsaturated Free fatty acid receptor biology.protein Heterologous expression Cell Division Polyunsaturated fatty acid |
| Zdroj: | Archives of Biochemistry and Biophysics. 376:399-408 |
| ISSN: | 0003-9861 |
| Popis: | We have cloned a Caenorhabditis elegans cDNA encoding a Δ12 fatty acid desaturase and demonstrated its activity by heterologous expression in Saccharomyces cerevisiae. The predicted protein is highly homologous both to the cloned plant genes with similar function and to the published sequence of the C. elegans omega-3 fatty acid desaturase. In addition, it conforms to the structural constraints expected of a membrane-bound fatty acid desaturase including the canonical histidine-rich regions. This is the first report of a cloned animal Δ12 desaturase gene. Expression of this cDNA in yeast resulted in the accumulation of 16:2 and 18:2 (linoleic) acids. The increase of membrane fluidity brought about by this change in unsaturation was measured. The production of polyunsaturated fatty acids in yeast cells and the concomitant increase in membrane fluidity was correlated with a modest increase in growth rate at low temperature and with increased resistance to ethanol and oxidative stress. |
| Databáze: | OpenAIRE |
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