Expression of human pancreatic secretory trypsin inhibitor in Saccharomyces cerevisiae
| Autor: | Ogawa Michio, Yaniamoto Tatsuo, Kikuchi Norihisa, Sato Takaaki, Yoshida Nobuo, Izumoto Yoshitaka, Matsubara Kenichi |
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| Rok vydání: | 1987 |
| Předmět: |
Signal peptide
Acid Phosphatase Genes Fungal genetic processes Saccharomyces cerevisiae PstI Pancreatic Juice Complementary DNA Genetics medicine Humans Cloning Molecular Promoter Regions Genetic Peptide sequence Pancreatic Secretory Trypsin Inhibitor Base Sequence biology Acid phosphatase General Medicine biochemical phenomena metabolism and nutrition Trypsin biology.organism_classification Molecular biology Genes Biochemistry Trypsin Inhibitor Kazal Pancreatic biology.protein bacteria Trypsin Inhibitors Plasmids medicine.drug |
| Zdroj: | Gene. 59:151-159 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(87)90323-4 |
| Popis: | Human pancreatic secretory trypsin inhibitor (PSTI) cDNA was expressed in Saccharomyces cerevisiae using the yeast acid phosphatase PH05 promoter. The product encoded by the PSTI-coding cDNA was correctly processed m yeast cells, and the PSTI molecules were efficiently secreted into the medium. The amino acid composition and the N-terminal amino acid sequence of the secreted PSTI molecules were identical to those of the authentic PSTI polypeptides from human pancreas, and the product exhibited trypsin-inhibitory activity. |
| Databáze: | OpenAIRE |
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