FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke
Autor: | Kamilla Malek, Katarzyna Bulat, Agnieszka Jasztal, Roman Pulyk, Jakub Dybas, Aneta Blat, Agnieszka Slowik, Tadeusz Popiela, Katarzyna M. Marzec, Magdalena Kaczmarska, Mateusz G. Adamski, Karolina Chrabaszcz |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Materials science
Science Optical spectroscopy 02 engineering and technology Microscopy Atomic Force Spectrum Analysis Raman Fibrin Article Imaging studies Brain Ischemia Chemometrics 03 medical and health sciences symbols.namesake Atomic force microscopy Medical and clinical diagnostics 0302 clinical medicine Microscopy Spectroscopy Fourier Transform Infrared Humans Fourier transform infrared spectroscopy Infrared spectroscopy Thrombectomy Multidisciplinary biology Resolution (electron density) Proteins Bioanalytical chemistry Thrombosis 021001 nanoscience & nanotechnology Lipids Characterization (materials science) Stroke Confocal microscopy Raman spectroscopy biology.protein symbols Medicine 0210 nano-technology 030217 neurology & neurosurgery Biomedical engineering |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | The significance and utility of innovative imaging techniques in arterial clot analysis, which enable far more detailed and automated analysis compared to standard methods, are presented. The examination of two types of human thrombi is shown, representing the main ischemic stroke etiologies: fibrin–predominant clot of large vessel origin and red blood cells–rich clot of cardioembolic origin. The synergy effect of Fourier–transform infrared spectroscopy (FTIR), Raman spectroscopy (RS) and atomic force microscopy (AFM) techniques supported by chemometrics in comparison with reference histological staining was presented. The main advantage of such approach refers to free–label and non–destructive quantitative imaging of clinically valid, biochemical parameters in whole sample (FTIR–low resolution) and selected regions (RS–ultra–high resolution). We may include here analysis of lipid content, its distribution and total degree of unsaturation as well as analysis of protein content (mainly fibrin and hemoproteins). The AFM studies enhanced the vibrational data, showed clearly shape and thickness of clot features as well as visualized the fibrin framework. The extraordinary sensitivity of FTIR and RS imaging toward detection and discrimination of clinically valid parameters in clot confirms its applicability in assessment of thrombi origin. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |