Myxobacterial biodiversity in an established oak-hickory forest and a savanna restoration site
| Autor: | Michael I. Kelrick, Dan Hite, Michael L. Lockhart, R. Brock Neil, Keesoo Lee |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Soil test Molecular Sequence Data Biodiversity complex mixtures Applied Microbiology and Biotechnology Microbiology Oak–hickory forest Quercus Myxobacteria RNA Ribosomal 16S Ecosystem Myxococcales Phylogeny Soil Microbiology Carya geography geography.geographical_feature_category Missouri biology Base Sequence Ecology General Medicine Standard methods biology.organism_classification RNA Bacterial Genes Bacterial visual_art visual_art.visual_art_medium Bark Whole cell |
| Zdroj: | Current microbiology. 50(2) |
| ISSN: | 0343-8651 |
| Popis: | The purpose of this study was to establish the myxobacterial biodiversity of an established oak-hickory forest and a savanna restoration site that has been cut and subsequently burned on four occasions between 1993 and 1998 in an attempt to restore the land to the native savanna ecosystem. Soil and bark samples were processed through standard methods specifically for myxobacteria and numbers and types of species were recorded for both locations. Species were identified through morphology of fruiting bodies, SDS-PAGE of whole cell protein profiles, and 16S rRNA gene sequences. Statistical analyses were employed and suggested that significantly greater numbers and types of myxobacteria are present on the bark of the trees in the established oak-hickory forest than the bark of trees in the savanna restoration site, while little difference in numbers and types of species were observed between the soil samples of the two locations. |
| Databáze: | OpenAIRE |
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