CD25+ B cells produced IL‐35 and alleviated local inflammation during experimental periodontitis
| Autor: | Yan Yu, Yakun Han, Chengcheng Yu, Liangjia Bi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Adoptive cell transfer Regulatory B cells Alveolar Bone Loss chemical and pharmacologic phenomena Inflammation T-Lymphocytes Regulatory 03 medical and health sciences 0302 clinical medicine Transforming Growth Factor beta medicine Humans IL-2 receptor Periodontitis General Dentistry B cell Tumor Necrosis Factor-alpha Chemistry Interleukin-17 TLR9 hemic and immune systems 030206 dentistry medicine.disease Interleukin-10 Toll-Like Receptor 4 medicine.anatomical_structure Otorhinolaryngology Toll-Like Receptor 9 030220 oncology & carcinogenesis Immunology TLR4 medicine.symptom |
| Zdroj: | Oral Diseases. 28:2248-2257 |
| ISSN: | 1601-0825 1354-523X |
| DOI: | 10.1111/odi.13939 |
| Popis: | BACKGROUND AND OBJECTIVE Host immunity is crucial during periodontal inflammations. B cells are considered to have a function of immunoregulation, and TLRs are considered to be crucial in this process. The present study illustrates the potential roles and rules of CD25+ B cells during periodontitis, especially its effect on regulating host IL-35 level and Th1, Th17, and Treg differentiation. MATERIAL AND METHODS The proportion of local and systemic CD25+ B cell subpopulations from periodontitis models were identified by flow cytometry. To illustrate further mechanism, B cells were cultured with a different type of TLR activators. Expression of IL-10, IL-35, and TGF-β was detected by ELISA and real-time PCR. We also set adoptive transfer models by using CD25+ B cells. Alveolar bone erosion, proportion of Th1, Th17, and Tregs, and levels of IFN-γ, TNF-α, IL-1β, and IL-17 were identified. RESULT Periodontitis induces more CD25+ B cell subpopulations and promotes their IL-10, IL-35, and TGF-βproduction. TLR activators enhanced Breg proliferation and function. LPS+CpG obviously induced more CD25+ B cell differentiation and production of IL-10, IL-35, and TGF-β. Adoptive transfer of CD25+ B cells reduces alveolar bone destruction and local Tregs, proportion, especially the local level of IFN-γ and IL-17. In addition, adoptive transfer of CD25+ B cells remedies the pathological change in the proportion of IL-1β and Th1/Th17 in local lesions. We did not find any significant difference in peripheral blood, regardless of group and detected items. CONCLUSION Results of the present study clarify that CD25+ B cells enlarged and produced more IL-10, IL-35 and TGF-β during periodontitis, activation of TLR4 and TLR9 played crucial roles in this process. Also, CD25+ B cells alleviated periodontal inflammation and alveolar bone resorption. Our findings further expanded the potential of B cells during periodontitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text