Adjuvants Induce Distinct Immunological Phenotypes in a Bovine Tuberculosis Vaccine Model
| Autor: | Philip J. Hogarth, G. S. Dean, Ida Rosenkrands, R. G. Hewinson, H. M. Vordermeier, Else Marie Agger, Peter Andersen, Daryan A. Kaveh |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Tuberculosis T-Lymphocytes medicine.medical_treatment Clinical Biochemistry Immunology In Vitro Techniques Biology Interferon-gamma Mice Immune system Adjuvants Immunologic Species Specificity medicine Animals Humans Immunology and Allergy Tuberculosis Vaccines Antigens Bacterial Immunity Cellular Mycobacterium bovis Immunogenicity Vaccine Research medicine.disease biology.organism_classification Antibodies Bacterial Virology Vaccination Phenotype Immunoglobulin G Liposomes BCG Vaccine Cattle Emulsions Tuberculosis vaccines Immunologic Memory Tuberculosis Bovine Adjuvant BCG vaccine |
| Zdroj: | ResearcherID |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | Tuberculosis (TB) remains one of the most important infectious diseases of humans and animals. Mycobacterium bovis BCG, the only currently available TB vaccine, demonstrates variable levels of efficacy; therefore, a replacement or supplement to BCG is required. Protein subunit vaccines have shown promise but require the use of adjuvants to enhance their immunogenicity. Using the protective mycobacterial antigen Rv3019c, we have evaluated the induction of relevant immune responses by adjuvant formulations directly in the target species for bovine TB vaccines and compared these to responses induced by BCG. We demonstrate that two classes of adjuvant induce distinct immune phenotypes in cattle, a fact not previously reported for mice. A water/oil emulsion induced both an effector cell and a central memory response. A cationic-liposome adjuvant induced a central memory response alone, similar to that induced by BCG. This suggests that water/oil emulsions may be the most promising formulations. These results demonstrate the importance of testing adjuvant formulations directly in the target species and the necessity of measuring different types of immune response when evaluating immune responses. |
| Databáze: | OpenAIRE |
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