Polyhexamethylene guanidine hydrochloride-based disinfectant: a novel tool to fight meticillin-resistant Staphylococcus aureus and nosocomial infections
| Autor: | Korami Dembélé, Lamine Diop, Richard Azinwi, Anne-Marie Maupertuis, Stephanie Mauler, Rose K. Nevry, Mathias K. Oulé, Lorraine Forbes, Tano Kablan, Anne-Marie Bernier |
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| Rok vydání: | 2008 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Microbiology (medical) Staphylococcus aureus Meticillin Disinfectant Microbial Sensitivity Tests Biology medicine.disease_cause Guanidines Microbiology chemistry.chemical_compound Salmonella Gram-Negative Bacteria Escherichia coli medicine Animals Humans Phenol coefficient Antibacterial agent Cross Infection Minimum bactericidal concentration General Medicine Antimicrobial Anti-Bacterial Agents chemistry Polyhexamethylene guanidine Pseudomonas aeruginosa Disinfectants medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 57:1523-1528 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.2008/003350-0 |
| Popis: | Polyhexamethylene guanidine hydrochloride (PHMGH), an antimicrobial biocide of the guanidine family, was tested for efficacy against quality-control strains of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella choleraesuis, meticillin-resistant S. aureus (MRSA) and Escherichia coli. Bactericidal activity against S. aureus, P. aeruginosa and Salmonella choleraesuis was determined using the official methods of analysis of the Association of Official Analytical Chemists, with modifications as recommended by the Canadian General Standards Board. For MRSA and E. coli, the MIC and minimal bactericidal concentration were determined using the broth dilution technique. The experiments were carried out at 20 °C under a range of conditions including varying PHMGH concentration (0.001–0.1 %), contact time (0.5–10 min) and water type (distilled, tap and hard water). The phenol coefficient values determined with S. aureus, Salmonella choleraesuis and P. aeruginosa were 7.5, 6.1 and 5, respectively. No matter what type of water was used to make the dilutions, PHMGH killed MRSA and E. coli at concentrations as low as 0.04 and 0.005 % (w/v), respectively, within 1.5 min. The mode of action of PHMGH was elucidated by transmission electron microscopy: the cell envelope was broken, resulting in cell content leakage into the medium. The ultimate aim of this study was to show that PHMGH can be used as an odourless, colourless, non-corrosive and harmless disinfectant for hospital and household facilities. |
| Databáze: | OpenAIRE |
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