Yersinia pestis Antigen F1 but Not LcrV Induced Humoral and Cellular Immune Responses in Humans Immunized with Live Plague Vaccine—Comparison of Immunoinformatic and Immunological Approaches
| Autor: | Yury V. Saltykov, Valentina A. Feodorova, Sergey S. Zaitsev, Maria A. Khizhnyakova, Vladimir L. Motin, Anna Lyapina |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Yersinia pestis Immunology lcsh:Medicine Biology immunoinformatics Epitope Article immune response 03 medical and health sciences 0302 clinical medicine Immune system Antigen vaccine Drug Discovery Pharmacology (medical) LcrV 030212 general & internal medicine correlates of immunity Pharmacology lcsh:R biology.organism_classification Virology plague peptide epitope mapping 030104 developmental biology Infectious Diseases Epitope mapping biology.protein Plague vaccine Antibody |
| Zdroj: | Vaccines Volume 8 Issue 4 Vaccines, Vol 8, Iss 698, p 698 (2020) |
| ISSN: | 2076-393X |
| DOI: | 10.3390/vaccines8040698 |
| Popis: | The recent progress in immunoinformatics provided the basis for an accelerated development of target-specific peptide vaccines as an alternative to the traditional vaccine concept. However, there is still limited information on whether the in silico predicted immunoreactive epitopes correspond to those obtained from the actual experiments. Here, humoral and cellular immune responses to two major Yersinia pestis protective antigens, F1 and LcrV, were studied in human donors immunized with the live plague vaccine (LPV) based on the attenuated Y. pestis strain EV line NIIEG. The F1 antigen provided modest specific cellular (mixed T helper 1 (Th1)/Th2 type) and humoral immune responses in vaccinees irrespective of the amount of annual vaccinations and duration of the post-vaccination period. The probing of the F1 overlapping peptide library with the F1-positive sera revealed the presence of seven linear B cell epitopes, which were all also predicted by in silico assay. The immunoinformatics study evaluated their antigenicity, toxicity, and allergenic properties. The epitope TSQDGNNH was mostly recognized by the sera from recently vaccinated donors rather than antibodies from those immunized decades ago, suggesting the usefulness of this peptide for differentiation between recent and long-term vaccinations. The in silico analysis predicted nine linear LcrV-specific B-cell epitopes however, weak antibody and cellular immune responses prevented their experimental evaluation, indicating that LcrV is a poor marker of successful vaccination. No specific Th17 immune response to either F1 or LcrV was detected, and there were no detectable serum levels of F1-specific immunoglobulin A (IgA) in vaccinees. Overall, the general approach validated in the LPV model could be valuable for the rational design of vaccines against other neglected and novel emerging infections with high pandemic potency. |
| Databáze: | OpenAIRE |
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