Rats treated with AZD2858, a GSK3 inhibitor, heal fractures rapidly without endochondral bone formation
| Autor: | Anna K. Sundgren-Andersson, Gregor Sisask, Richard Marsell, Sune Larsson, Kenneth B. Jonsson, Östen Ljunggren, Olle Nilsson |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Histology Physiology Endocrinology Diabetes and Metabolism Bone healing Piperazines Rats Sprague-Dawley Glycogen Synthase Kinase 3 Bone Density Osteogenesis Oral administration GSK-3 Internal medicine Animals Medicine Femur Bony Callus Quantitative computed tomography Bone regeneration Protein Kinase Inhibitors Endochondral ossification Fracture Healing Sulfonamides medicine.diagnostic_test business.industry Cartilage Mesenchymal stem cell Organ Size Biomechanical Phenomena Rats Surgery Radiography medicine.anatomical_structure Endocrinology Pyrazines Female business Femoral Fractures |
| Zdroj: | Bone. 54:126-132 |
| ISSN: | 8756-3282 |
| Popis: | Fracture healing is a complex interplay between endochondral and intramembranous bone formation processes. The canonical Wnt/β-catenin pathway enhances new bone formation and may play a role in fracture healing. Glycogen synthase kinase 3β (GSK3β) is a key regulator of β-catenin degradation. In this study, we investigate the effects of AZD2858, an orally bioactive GSK3 inhibitor, on fracture healing. Femoral fractures were produced in rats after the insertion of a femoral nail. The rats were treated with oral administration of AZD2858 at a dose of 30 μmol/kg (20mg/kg) daily for up to 3 weeks, while control animals were administered vehicle. At 4days, and at 1, 2 and 3 weeks, histological analysis was performed, and at the 2 and 3 week time points, we performed peripheral quantitative computed tomography (pQCT), X-rays, and four-point bending tests. Peripheral QCT showed an increase in both mineral density (of 28% at 2 weeks and 38% at 3weeks) and mineral content (of 81% at 2 weeks and 93% at 3 weeks) in the calluses from AZD2858 treated animals as compared to vehicle treated animals. Histological analysis demonstrated that rats treated with GSK3 inhibitor healed their fractures rapidly, but without the pre-formation of cartilage tissue. Furthermore, four-point bending tests of fractured femora from animals treated for 2 and 3 weeks showed an increase in strength in treated animals compared to their vehicle-treated controls. In conclusion, AZD2858, a potent GSK3 inhibitor, has a substantial impact on fracture healing. The fractures healed with a bony callus without an obvious endochondral component, suggesting that AZD2858 drives mesenchymal cells into the osteoblastic pathway. This leads to direct bone repair in an unstable fracture milieu. |
| Databáze: | OpenAIRE |
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