Oxidative biodegradation of phosphorothiolates by fungal laccase
| Autor: | L. Leibovitz-Persky, Adam Vincze, Haim Leader, Gabriel Amitai, Ishai Rabinovitz, Gali Sod-Moriah, Yitzhak Hadar, Rachel Adani, D. Friesem, Benny Chefetz |
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| Rok vydání: | 1998 |
| Předmět: |
Insecticides
Biophysics Pleurotus Biochemistry Diisopropyl-Amiton Substrate Specificity Phosphorothiolate chemistry.chemical_compound Enzymatic oxidation Organophosphorus Compounds Structural Biology Enzymatic hydrolysis Genetics medicine Organic chemistry Benzothiazoles Molecular Biology Nerve agent Laccase O-Isobutyl S-[N N-diethylaminoethyl]methylphosphonothiolate Oxidase test ABTS Chromatography biology Organothiophosphorus Compounds Cell Biology Pleurotus ostreatus Chaetomium Biodegradation biology.organism_classification O-Ethyl S-[N N-diisopropylaminoethyl]methylphosphonothiolate Kinetics 2 2′-Azinobis(3-ethylbenzthiazoline-6-sulfonate) Biodegradation Environmental chemistry Sulfonic Acids Oxidoreductases Oxidation-Reduction medicine.drug |
| Zdroj: | FEBS letters. 438(3) |
| ISSN: | 0014-5793 |
| Popis: | Organophosphorus (OP) insecticides and nerve agents that contain P-S bond are relatively more resistant to enzymatic hydrolysis. Purified phenol oxidase (laccase) from the white rot fungus Pleurotus ostreatus (Po) together with the mediator 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) displayed complete and rapid oxidative degradation of the nerve agents VX and Russian VX (RVX) and the insecticide analog diisopropyl-Amiton with specific activity: k(sp) = 2200, 667 and 1833 nmol min(-1) mg(-1), respectively (pH 7.4, 37 degrees C). A molar ratio of 1:20 for OP/ABTS and 0.05 M phosphate at pH 7.4 provided the highest degradation rate of VX and RVX. The thermostable laccase purified from the fungus Chaetomium thermophilium (Ct) in the presence of ABTS caused a 52-fold slower degradation of VX with k(sp) = 42 nmol min(-1) mg(-1). The enzymatic biodegradation products were identified by 31P-NMR and GC/MS analysis. |
| Databáze: | OpenAIRE |
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